H-键型热响应性精神分裂性共聚物:与其母聚合物的相位过渡相关性以及改善的蛋白质联合组装能力
Jiahui Lai1, Jialin Sun1, Chen Li1
1Tianjin Key Laboratory of Biomedical Materials, Institute of Biomedical Engineering, Chinese Academy of Medical Sciences & Peking Union Medical College, Tianjin 300192, China.
Journal of colloid and interface science
|July 30, 2023
概括
这项研究合成了新型精神分裂性共聚物,具有可调节的热响应性行为,以实现高效的蛋白质联合组装. 这些智能聚合物在生物医学应用中显示出潜力,同时保持蛋白质生物活性并表现出无毒性.
科学领域:
- 聚合物化学 聚合物化学
- 材料科学 材料科学 材料科学
- 生物材料是一种生物材料.
背景情况:
- 精神分裂性共聚合物是具有温度依赖结构的智能聚合物,但预测它们的相位过渡和理解应用是具有挑战性的.
- 现有的研究缺乏明确的原则来预测从母聚合物中预测精神分裂性共聚合物相位过渡温度.
- 精神分裂性共聚合物的应用仍未得到充分探索,阻碍了它们的开发和使用.
研究的目的:
- 合成和描述具有明显热响应行为 (UCST-LCST,LCST-UCST或LCST) 的新型精神分裂共聚物.
- 研究这些共聚合物与蛋白质的联合组装,重点关注加载动力学和生物活性保护.
- 建立精神分裂性共聚合物及其母聚合物相位过渡之间的初步相关性.
主要方法:
- 通过使用P(AAm-co-NAS-co-AAc) 前体和NIPAm/AAm混合物,通过RAFT聚合合成精神分裂性共聚合物的合成.
- 在水溶液中的热响应转换 (UCST和LCST) 的表征.
- 与蛋白质进行联合组装研究,以评估负载效率,动力学和生物活性.
- 使用NIH 3T3和MCF-7细胞系进行体外细胞毒性测定.
主要成果:
- 成功合成了表现出各种热响应过渡的精神分裂共聚物 (UCST-LCST,LCST-UCST,LCST).
- 证明了高效的蛋白质联合组装,具有超高的蛋白质负载能力.
- 在联合组装后确认了蛋白质生物活性的保存.
- 建立了预测相位转换的初步相关性.
- 所有合成的材料都没有对测试的细胞系产生毒性.
结论:
- 精神分裂性共聚合物是蛋白质联合组装的有效平台,提供高负载并保持生物活性.
- 合成的材料为软合体和组装系统提供了有希望的前景.
- 这项工作为设计用于生物医学应用的新型精神分裂聚合物提供了指导.
- 开发的共聚物是无毒的,增强了它们在体内使用的潜力.
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