基于溶剂的同质液液微提取可切换的水友性新应用,通过LC-MS/MS在血中分析合成大麻素
André Luis Fabris1, Aline Franco Martins2, Jose Luiz Costa3
1School of Pharmaceutical Sciences, University of São Paulo, São Paulo, SP 05508-000, Brazil.
Journal of pharmaceutical and biomedical analysis
|July 30, 2023
概括
这项研究引入了一种绿色分析方法,即可切换的水性溶剂基同质液体-液体微提取 (SHS-HLLME),用于检测血中的合成大麻素. 这种环保技术为这些强效化合物提供了可靠的分析.
科学领域:
- 分析化学 分析化学
- 法医毒理学 法医毒理学
背景情况:
- 合成大麻素是一种越来越多的强烈精神活性物质.
- 准确的检测需要从等离子体等生物矩阵中有效地准备样本.
- 现有的方法往往缺乏环保属性,与绿色分析毒理学原则形成鲜明对比.
研究的目的:
- 开发和验证一种新的,环保的合成大麻素样品制备技术.
- 为此目的,利用可切换的水友性溶剂基同质液-液微提取 (SHS-HLLME).
- 优化SHS-HLLME程序以提高效率和环境可持续性.
主要方法:
- 使用可切换的水友性溶剂为基础的同质液体-液体微提取 (SHS-HLLME).
- 优化包括调整SHS类型,溶剂体积,pH (使用NaOH) 和提取时间.
- 在血样本上进行了蛋白质沉,溶剂蒸发和液体-液体提取步骤.
主要成果:
- 优化的SHS-HLLME程序在0.1-10 ng/mL范围内显示出高线性 (R2 ≥0.99).
- 实现了低检测值 (0.01-0.08 ng/mL) 和定量值 (0.1 ng/mL).
- 对于31种合成大麻素,仅使用300μL血,获得了令人满意的恢复 (36-56.7%) 和工艺效率 (18.5-148.4%).
结论:
- SHS-HLLME是作为一种可靠和环保的替代方案来分析合成大麻素.
- 该方法有效地从血中提取低度的各种合成大麻素.
- 这种技术符合绿色分析毒理学原则,减少对有毒有机溶剂的依赖.
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