功能多样化的前端脱酶在类Annelida中广泛存在
Marc Ramos-Llorens1, Francisco Hontoria1, Juan C Navarro1
1Instituto de Acuicultura Torre de la Sal (IATS), CSIC, 12595 Ribera de Cabanes, Castellón, Spain.
概括
类动物,包括地和海洋,可以合成必需的欧米茄-3长链多不和脂肪酸 (ω3 LC-PUFA). 这些虫拥有关键的酶,使得这些重要的脂肪酸在各种生态系统中的多功能生产成为可能.
科学领域:
- 海洋生物学 海洋生物学
- 生物化学 生物化学
- 基因组学就是基因组学.
背景情况:
- 水生单细胞生物被认为是omega-3长链多不和脂肪酸 (ω3 LC-PUFA) 的主要生产者.
- 最近的发现表明,无脊椎动物,像状动物一样,拥有甲基末端脱酶用于 ω3 LC-PUFA 合成,这表明海洋生产范围更广.
- 参与化物LC-PUFA生物合成的全谱酶仍然在很大程度上未被描述.
研究的目的:
- 为了研究Annelida系内的前端脱酶基因库.
- 为了在代表性类物种中对这些脱酶酶进行功能性表征.
- 了解水生和陆生环境中类动物中LC-PUFA合成的进化多样化.
主要方法:
- 在类类 (Polychaeta,Clitellata) 中检查了前端脱酶基因.
- 利用异质酵母表达系统来描述酶功能.
- 分析的基因补充保护跨类的分类学群.
主要成果:
- 化物至少有两个前端脱酶,具有Δ5和Δ6Δ8的区域选择性.
- 这些酶有助于将C18前体转化为必不可少的LC-PUFA,如eicosapentaenoic和arachidonic酸.
- 对于LC-PUFA合成的已识别的基因补充物在化物多样性中被保留,尽管观察到功能多样化.
结论:
- 类动物拥有一套完整的脱酶基因,用于合成关键的LC-PUFA,除了多可沙赫酸.
- 虫前端脱酶功能已经显著多样化,适应陆地和水生息地.
- 这项研究扩大了已知的 ω3 LC-PUFA 生产者超出单细胞生物的范围,突出了类动物的重大贡献.
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