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波利亚可可的多糖化物改善了肠道屏障的功能,并维持了小鼠的肠道平衡

Yuting Duan¹, Jiajing Huang¹, Mingjie Sun¹

¹School of Pharmacy, Anhui University of Chinese Medicine, China.

International journal of biological macromolecules

|July 30, 2023

概括

孔雀子多糖 (PCP) 通过加强身体,生化和免疫防御来增强小鼠的肠道屏障功能. 这些发现表明PCP可以成为改善肠道健康的功能性食物.

科学领域:

胃肠病学和肠道健康
功能性食品和营养保健品
免疫学和微生物学

背景情况:

肠道的功能对人类整体健康至关重要.
Poria cocos是一种功能性食用真菌,在亚洲用于胃肠道调节.
需要阐明Poria cocos多糖 (PCP) 对肠道的特定影响.

研究的目的:

研究 Poria cocos 聚糖 (PCP) 对小鼠肠粘膜屏障功能的影响.
为了描述提取的PCP的化学特性.
评估PCP对肠道生理,物理,生化,免疫和微生物屏障的影响.

主要方法:

波利亚可可斯多糖的提取和表征 (PCP).
通过食向小鼠注射PCP.
评估肠道屏障功能,包括基因表达 (Occludin,ZO-1,MUC2,β-defensin,SIgA,Wnt/β-Catenin,Lrp5),血清标志物 (内毒素,DAO,D-乳酸盐),肠道MPO,细胞因子水平 (IL-2,IL-4,IL-6,IL-10,TGF-β,IFN-γ),短链脂肪酸 (SCFA) 和肠道微生物群组成.

主要成果:

PCP的分子量为11.583kDa,主要由曼诺糖,D-葡萄糖胺化物,葡萄糖,银糖和糖组成.
PCP显著改善了肠道生理状态,加强了物理屏障 (上调奥克卢丁,ZO-1;下调内毒素,DAO,D-乳酸盐,MPO),增强了生化屏障 (上调MUC2,β-防御素,SIgA),并调节了免疫屏障 (增加关键细胞因子).
PCP增加了SCFA,改变了肠道微生物组成,并上调了Wnt/β-Catenin和Lrp5的表达,表明肠道屏障功能的维持.

结论:

在小鼠中,Poria cocos 多糖 (PCP) 有效地增强了肠粘膜屏障功能的多个方面.
PCP的机制包括加强物理和生化障碍,调节免疫反应,改变肠道微生物群.
PCP显示出作为一种功能性食品成分的潜力,可以促进肠道健康和宿主福祉.

关键词:

肠道菌群是什么意思肠道菌群肠道屏障的功能肠道屏障的功能波利亚可可斯的多糖化物.结构分析结构分析在Wnt/β-catenin路径中.

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