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一种修饰的Anemarrhena asphodeloides多糖复合物通过NF-κB和MAPK信号通路增强免疫活性

Shaojie Zhang¹, Qian Zhang², Chong Li³

¹Guangdong Provincial Key Laboratory of Research and Development of Natural Drugs, School of Pharmacy, Guangdong Medical University, Dongguan 523808, China.

International journal of biological macromolecules

|July 30, 2023

概括

亚尼马雷纳亚斯福德洛伊德多糖化物-复合物 (AAP-Zn) 通过促进细胞和细胞因子产生来增强免疫反应. 这种复合体通过NF-κB和MAPK通路激活巨细胞,显示出作为免疫增强剂的潜力.

科学领域:

生物化学生化学
免疫学免疫学免疫学
材料科学材料科学材料科学

背景情况:

亚尼马雷纳亚斯福德化物多糖 (AAP70-1) 具有免疫调节的特性.
对于生物医学应用,需要进一步增强AAP70-1的免疫调节作用.

研究的目的:

为了合成一个Anemarrhena asphodeloides多糖化合物-复合物 (AAP-Zn).
研究AAP-Zn在激活巨细胞和增强免疫反应中的机制.

主要方法:

使用ZnCl2修饰合成AAP-Zn.
AAP-Zn的结构特征,包括含量分析.
对斑马鱼胚胎的免疫调节作用的评估,包括化,细胞因子mRNA表达 (TNF-α,IL-6,IL-1β,IL-18),氧化 (NO) 和反应性氧物种 (ROS) 的产生.
研究细胞内信号通路 (NF-κB和MAPK).

主要成果:

AAP-Zn保留了与AAP70-1的结构相似性,含量为0.2%,通过协调键结合.
无论是AAP70-1还是AAP-Zn,都在斑马鱼胚胎中改善了免疫调节效应.
AAP-Zn增强了细胞化,上调了关键细胞因子mRNA的表达,并增加了NO和ROS的产生.
由AAP-Zn激活巨细胞涉及NF-κB和MAPK信号通路.

结论:

与AAP70-1相比,AAP-Zn是一种新的复合物,具有增强的免疫调节特性.
作用机制包括通过NF-κB和MAPK信号激活巨细胞.
作为生物医学和功能性食品的潜在免疫增强剂,AAP-Zn显示出希望.

关键词:

亚尼马雷纳 (Anemarrhena asphodeloides Bunge) 是一种的植物.免疫调节活动.MAPKs 信号通路的信号通路.通过NF-κB信号通路传递信号.斑马鱼是一种斑马鱼.的修改是的修改.

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