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对CHROMagar Candida Plus介质用于酵母的推定识别和MALDI-TOF MS识别的评估

Constanza Giselle Taverna¹, Matías Ezequiel Vivot¹, Bárbara Abigail Arias¹

¹Departamento Micología, Instituto Nacional de Enfermedades Infecciosas "Dr. Carlos G. Malbrán", Buenos Aires, Argentina.

|July 30, 2023

概括

新的CHROMagarTM Candida Plus介质有助于快速识别酵母,正确识别常见的物种,如Candida albicans和Candida tropicalis. 它还选Candida auris和Candida glabrata,但为了准确性,建议使用MALDI-TOF进行确认.

科学领域:

临床微生物学临床微生物学
菌类学菌类学是指菌类学.
诊断实验室科学诊断实验室科学

背景情况:

准确的酵母鉴定对于临床环境中有效的抗真菌治疗至关重要.
CHROMagarTM Candida Plus是一种新型介质,用于酵母的推定识别.
矩阵辅助激光吸附/电离-飞行时间 (MALDI-TOF) 质谱是关键的识别方法.

研究的目的:

为了评估CHROMagarTM Candida Plus的性能,以推测酵母菌的鉴定.
将识别结果与MALDI-TOF质谱法进行比较.
评估化温度和时间对MALDI-TOF识别分数的影响.

主要方法:

使用CHROMagarTM Candida Plus.测试了来自60个物种的303个酵母菌株.
通过使用MALDI-TOF在30°C和37°C,24小时和72小时的化时间,进一步确定了215个菌株.
推测和确认的鉴定被比较.

主要成果:

CHROMagarTM Candida Plus显示了对Candida albicans复合体,Candida tropicalis和Pichia kudriavzevii (Candida krusei) 的正确的推定识别.
该媒介确定了所有Candida auris和Candida glabrata菌株,尽管其他一些物种被误认为是这些.
在测试条件下,MALDI-TOF正确识别了94%的菌株 (物种或复杂水平).
化时间影响MALDI-TOF得分在37°C,但不影响30°C.

结论:

CHROMagarTM Candida Plus在推测关键酵母菌种的识别方面表现良好.
该介质对于查Candida auris和Candida glabrata非常有价值.
使用MALDI-TOF等方法确认酵母鉴定对于临床准确性至关重要.

关键词:

马格里亚人参虫 (Candida) 的生物这就是CANDIDA PLUS的CHROMagar.这是一种Candida Candida.它们是Candida auris.马尔迪 - 托夫

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相关概念视频

MALDI-TOF Mass Spectrometry

MALDI-TOF Mass Spectrometry

Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.
Matrix-assisted laser desorption ionization (MALDI) is a commonly...