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Updated: Jul 20, 2025

Protein WISDOM: A Workbench for In silico De novo Design of BioMolecules
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Protein WISDOM: A Workbench for In silico De novo Design of BioMolecules

Published on: July 25, 2013

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重新设计 metionyl-tRNA合成酶以适应性地形平整和实验为β-methionine活动

Vaitea Opuu1, Giuliano Nigro1, Christine Lazennec-Schurdevin1

  • 1Laboratoire de Biologie Structurale de la Cellule (CNRS UMR7654), Ecole Polytechnique, Institut Polytechnique de Paris, Palaiseau, France.

Protein science : a publication of the Protein Society
|July 30, 2023
PubMed
概括

计算式蛋白质设计使得非正规氨基酸的氨基-tRNA合成酶的工程成为可能. 这种进步使得蛋白质工程中的新蛋白质结构和构建块成为可能.

关键词:
这是Proteus软件.在X射线晶体学.遗传密码扩张 扩张分子建模分子建模

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One of the unique features of tRNA is the presence of modified bases. In some tRNAs, modified bases account for nearly 20% of the total bases in the molecule. Altogether, these unusual bases protect the tRNA from enzymatic degradation by RNases.
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Base complementarity between the three base pairs of mRNA codon and the tRNA anticodon is not a failsafe mechanism. Inaccuracies can range from a single mismatch to no correct base pairing at all. The free energy difference between the correct and nearly correct base pairs can be as small as 3 kcal/ mol. With complementarity being the only proofreading step, the estimated error frequency would be one wrong amino acid in every 100 amino acids incorporated. However, error frequencies observed in...
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