来自干细胞的外体作为在实验动物中治疗分裂性肝纤维化的潜在疗法
Asmaa R Ellakany1, Hanan El Baz2, Zeinab S Shoheib1
1Medical Parasitology Department, Faculty of Medicine, Tanta University, Tanta, Egypt.
Pathogens and global health
|July 31, 2023
概括
介质干细胞 (MSCs) 衍生的外体在治疗因杆菌病引起的肝纤维化方面表现有前途. 在小鼠中,将外体与普拉齐特 (PZQ) 结合起来,证明了协同作用的抗纤维和抗炎作用.
科学领域:
- 寄生虫学的寄生虫学
- 再生医学是一种再生医学.
- 免疫学 免疫学 免疫学
背景情况:
- 湿病是一种被忽视的热带疾病,由于卵诱导的颗粒瘤形成和肝纤维化,导致显著的发病率和死亡率.
- 介质干细胞 (MSCs) 衍生的外体提供了MSCs治疗肝纤维化的更安全的替代方案.
研究的目的:
- 为了研究MSCs衍生的外体的治疗潜力,在治疗分裂性肝纤维化.
- 为了评估MSCs衍生的外体和PZQ (PZQ) 对分裂性肝纤维化的联合作用.
主要方法:
- 从骨髓MSCs中分离和表征外体.
- 试验感染了85只小鼠,分为对照,PZQ,EXO和PZQ+EXO治疗组.
- 通过对增殖细胞核抗原 (PCNA) 和核因子-κB (NF-κB) 的组织病理学检查和免疫组织化学分析来评估治疗疗效.
主要成果:
- 在感染的小鼠中,MSCs衍生的外体显著减少了肝脏颗粒瘤和纤维化.
- 用外体和组合疗法的治疗提高了PCNA表达的调节,并降低了NF-κB表达.
- 与单个治疗相比,PZQ和外体组合 (PZQ+EXO) 显示出增强的治疗效果.
结论:
- 由MSCs衍生出的外体是治疗分裂性肝纤维化的一个有前途的治疗剂.
- 与PZQ和MSCs衍生的外体组合疗法表现出协同的抗纤维和抗炎作用.
- 在这种情况下,需要进一步的研究来阐明MSCs衍生的外体的功能成分和作用机制.
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