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致命的COVID-19的定量多器官蛋白质学揭示了除了炎症之外的组织特异性影响

Lisa Schweizer¹, Tina Schaller², Maximilian Zwiebel¹

¹Department of Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Martinsried, Germany.

EMBO molecular medicine

|July 31, 2023

概括

这项研究揭示了SARS-CoV-2感染引起的广泛器官损伤,影响肺部,脏,肝脏和大脑. 蛋白质组学确定了COVID-19独特的损伤模式和炎症,建议针对性的疗法.

科学领域:

生物化学生化学
病理学病理学病理学
免疫学免疫学免疫学

背景情况:

SARS-CoV-2 感染导致显著的发病率和死亡率.
系统性和器官特异性损伤机制仍然不完全理解.
对于COVID-19的器官损伤,几乎没有针对性的,全系统的蛋白质组研究存在.

研究的目的:

综合分析SARS-CoV-2感染引起的系统性和器官特异性蛋白质组变化.
区分一般炎症和真正的特定组织损伤模式.
为了确定COVID-19器官损伤的潜在治疗点.

主要方法:

开发一个并行质谱 (MS) 蛋白质组学工作流程.
数以百计感染病毒的形式固化嵌 (FFPE) 组织的定量蛋白质组分析.
系统范围的,无针对性的分析,以捕捉广泛的分子变化.

主要成果:

所有由系统性炎症分子主导的组织的初始反应.
肺部蛋白质组变化模仿了非COVID-19患者中观察到的扩散膜损伤 (DAD).
在脏,肝脏,淋巴和血管系统中观察到显著的器官特异性蛋白质组变化.
大脑表现出二次炎症效应,包括神经递质受体变化和髓降解.

结论:

感染SARS-CoV-2会导致系统性炎症以外的复杂的多器官损伤.
蛋白质基因分析揭示了与COVID-19相关的器官损伤模式.
这些发现支持开发针对COVID-19的器官特定治疗策略.

关键词:

在 COVID-19 疫情中,质谱测量质谱测量质谱测量质谱测量质量测量质谱测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量质量测量病理学的病理学蛋白质组学蛋白质组学病毒病毒病毒病毒

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