诱导脏相关的基因程序通过SALL1在卵巢试验中的合作选择
Magdalena Schindler1,2, Marco Osterwalder3,4,5, Izabela Harabula6
1Gene Regulation & Evolution, Max Planck Institute for Molecular Genetics, Berlin 14195, Germany.
Development (Cambridge, England)
|July 31, 2023
概括
基因表达的变化驱动了特征的进化. 研究人员发现,子中SALL1基因的增强因子活性发生变化,其表达方式被采用,导致卵巢细胞变得男性化,并证明了进化创新的机制.
科学领域:
- 进化生物学是进化的生物学.
- 发育生物学是发展生物学.
- 遗传学 遗传学 是一个
背景情况:
- 现型创新源于基因表达的变化,但根本的机制尚未完全理解.
- 了解基因表达变化如何影响特征进化对于进化生物学来说至关重要.
研究的目的:
- 研究雌性子中男性化卵巢的发展背后的分子机制.
- 确定基因表达变化和表观遗传修饰在特征进化中的作用.
主要方法:
- 从和小鼠获得的表观遗传和转录数据的整合分析.
- 染色体形状捕获以研究SALL1位置的3D组织.
- 跨物种报告员测试以评估增强剂活性.
- 在转基因小鼠中的过度表达实验.
主要成果:
- 鉴定出SALL1基因表达的共选是分子卵巢形成的关键.
- 萨尔1位点的3D组织被保留,但增强器活动在进化过程中有所分离.
- 分子特异性增强剂可以激活泌尿器官组织中的转录.
- 在小鼠中,SALL1过度表达诱导了与脏相关的基因程序,这是卵子试验的标志性特征.
结论:
- 增强剂活性导致基因表达合作的变化为新型特征的进化提供了一个合理的机制.
- 这项研究阐明了调节元素进化在塑造表型多样性的作用.
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