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从人类脱皮叶状牙 (SHED) 的干细胞中获得的iPSCs中区分外围感官神经元

Nathalia C Oliveira^1,2, Fabiele B Russo¹, Patricia C B Beltrão-Braga^1,2

¹Disease Modeling Laboratory, Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil.

Frontiers in cell and developmental biology

|July 31, 2023

概括

研究人员开发了一种新方法,可以从婴儿牙中获得的干细胞中创建外围感官神经元. 这一突破为研究和治疗神经系统疾病提供了有希望的途径.

科学领域:

干细胞生物学干细胞生物学
神经科学是一个神经科学.
发育生物学是发展生物学.

背景情况:

周围神经系统 (PNS) 的感觉变化与各种疾病有关.
诱导多能干细胞 (iPSC) 技术对于产生用于治疗应用的感觉神经元至关重要.
现有的iPSC协议经常使用纤维细胞,这些纤维细胞与中枢神经系统 (CNS) 具有共同的胚胎起源,可能会影响衍生的PNS细胞.

研究的目的:

建立一种用于区分外围感官神经元 (PSN) 与来自人类脱皮叶状牙 (SHED) 的干细胞的新协议.
利用SHED的神经起源产生PNS特异细胞,克服中枢神经系统衍生的iPSCs的局限性.
通过提供新的细胞来源,使未来的PNS病理研究成为可能.

主要方法:

来自SHED的iPSCs的生成.
使用双SMAD抑制,将SHED衍生的iPSC分化为PSN.
应用神经元分化因子,包括人工神经变因子和状细胞衍生分子.

主要成果:

成功开发了第一个区分神经和PNS细胞与SHED衍生的iPSCs的协议.
证明了使用SHED作为PNS相关细胞生成源的可行性.
建立了一种尊重所需细胞类型本体遗传起源的方法.

结论:

这项研究提出了一个突破性的协议,用于从SHED衍生的iPSC生成PNS细胞.
这种新方法利用了适合PNS发育的胚胎起源 (神经) 的细胞.
这一进展为研究和治疗PNS疾病带来了巨大的潜力.

关键词:

棚屋棚屋是一个棚屋.iPSC 的差异化差异化.神经细胞是神经顶细胞.周围神经系统周围神经系统周边感官神经元是周边感官神经元.

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