冥想与端粒动态的关联:在健康成年人中进行的病例对照研究
Nirodhi Namika Dasanayaka1, Nirmala Dushyanthi Sirisena2, Nilakshi Samaranayake3
1Research Promotion and Facilitation Centre, Faculty of Medicine, University of Colombo, Colombo, Sri Lanka.
Frontiers in psychology
|July 31, 2023
概括
长期冥想与更长的端粒长度 (TL) 和参与端粒维护的关键基因的表达增加有关. 这种心身实践可以通过积极影响端粒动态来促进健康的衰老.
科学领域:
- 遗传学 是一个遗传学.
- 分子生物学分子生物学
- 衰老研究研究 衰老研究
背景情况:
- 端粒保护染色体末端,并且随着年龄的增长而缩短,与与年龄相关的疾病有关.
- 冥想是一种心身实践,对端粒动态有潜在的影响.
- 以前的研究将冥想与更高的端粒酶水平和改善生活质量联系起来.
研究的目的:
- 调查长期冥想与端粒长度 (TL) 之间的关联.
- 检查冥想对人类端粒酶逆转录酶 (hTERT) 和人类端粒酶RNA (hTR) 基因表达的影响.
- 评估冥想对hTERT基因促进子区域甲基化的影响.
主要方法:
- 测量端粒长度的定量PCR.
- 反转录酶PCR用于hTERT和hTR的基因表达分析.
- 双硫酸盐特异性PCR和桑格测序用于hTERT促进剂甲基化分析.
- 统计分析包括t测试,相关性和回归.
主要成果:
- 与非冥想者相比,冥想者表现出较长的相对端粒长度 (TL) (p=0.020).
- 长期冥想持续时间显著预测了TL (p=0.003).
- 冥想者对hTERT (p=0.020) 和hTR (p=0.029) 基因的相对表达更高.
- 在冥想者中观察到hTERT促进器区域的低甲基化 (p<0.001).
- 在hTERT促进物甲基化和hTERT基因表达 (p=0.001) 和冥想持续时间 (p=0.001) 之间发现了负相关性.
结论:
- 冥想与更长的端粒长度和与端粒维护相关的有利基因表达模式有关.
- 冥想可以通过表观遗传修饰来影响端粒动力学,例如减少hTERT促进物甲基化.
- 这些发现表明,冥想是一种生活方式实践,具有促进健康衰老的多层次益处.
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