富士多糖在免疫抑制小鼠模型中通过调节肠道微生物群组成来提高免疫力
Ran Tu1, Cheng Zhou1, Wenfeng Huang1
1Medical Laboratory of Jingmen People's Hospital, Jingchu University of Technology Affiliated Central Hospital, Jingmen, Hubei, China.
Heliyon
|July 31, 2023
概括
来自传统中医药的富士多糖增强了肠道微生物群,并提高了免疫抑制小鼠的免疫力. 这些化合物调节炎症性细胞因子和肠道细菌,改善免疫功能,并提供对传统医学机制的见解.
科学领域:
- 免疫学 免疫学 免疫学
- 微生物学 微生物学
- 药理学 药理学是指药理学的学科.
背景情况:
- 富士 (Aconitum carmichaelii Debx) 是一个传统的中国医学.
- 富士多糖是具有潜在免疫调节作用的生物活性化合物.
- 富士多糖对肠道微生物群和免疫力的确切机制尚不清楚.
研究的目的:
- 为了研究富士多糖对肠道微生物群组成的影响.
- 评估富士多糖在免疫抑制小鼠模型中对免疫功能的影响.
- 阐明富士多糖的免疫调节作用背后的分子机制.
主要方法:
- 环胺诱导的免疫抑制小鼠模型.
- 通过气色谱-质谱分析短链脂肪酸 (SCFA).
- 使用16S rRNA基因测序进行肠道微生物群分析.
- 通过qRT-PCR测量炎症标志物 (NF-κB,IL-6,TNF-α,iNOS,COX-2) 的结果.
- 评估肠道屏障蛋白质 (occludin,ZO-1) 通过西方的 blot.
- 免疫细胞分析包括白细胞计数和调节性T细胞群 (流细胞计).
主要成果:
- 富士多糖在内增加了有益的短链脂肪酸 (SCFA).
- 观察到肠道微生物群组成的显著变化,病原生物的丰富性下降,有益细菌增加.
- 富士多糖改善了脏和胸腺指数,白细胞计数和淋巴细胞数量.
- 恢复由环胺抑制的炎症性细胞因子表达 (NF-κB,IL-6,iNOS) 和肠道屏障蛋白水平 (occludin,ZO-1).
- 在细胞因子表达和肠道微生物群组成之间发现了显著的相关性.
结论:
- 富士多糖化物调节肠道微生物群的组成和SCFA的产生.
- 这些多糖增强免疫细胞的数量和功能在免疫抑制小鼠.
- 富士多糖通过调节炎性细胞因子和肠道屏障完整性来恢复免疫平衡.
- 这些发现突显了富士多糖作为免疫调节剂的潜力,并为中国传统医学提供了机械洞察力.
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