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ZNF480 影响低度质瘤患者的预后,发病和免疫微环境

Qingyun Zhu¹, Zhendong Liu², Xingbo Cheng²

¹People's Hospital of Henan University, Henan Provincial People's Hospital, Microbiome Laboratory, Zhengzhou 450003, Henan Province, China.

|July 31, 2023

概括

指蛋白480 (ZNF480) 在低级质瘤 (LGG) 中表达高,与患者生存率差相关. 这项研究揭示了ZNF480在促进免疫支持瘤微环境中的作用,表明了潜在的治疗点.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
免疫学免疫学免疫学

背景情况:

指480 (ZNF480) 在恶性瘤,特别是低度质瘤 (LGG) 中的作用仍然在很大程度上未被探索.
了解LGG病变的新型分子参与者对于开发有效的治疗策略至关重要.

研究的目的:

调查ZNF480在LGG病理过程中的重要性.
探索ZNF480表达和患者预后之间的相关性.
阐明ZNF480在瘤微环境中的作用及其与免疫细胞和检查点的关系.

主要方法:

对大规模公共数据库样本和体外实验进行分析.
逆转录定量实时聚合酶连锁反应 (RT-qPCR) 和免疫组织化学用于表达分析.
基因组丰富分析 (GSEA) 和斯皮尔曼相关性分析用于途径和免疫学关联.

主要成果:

发现ZNF480在LGG的mRNA和蛋白质水平上都高度表达.
高ZNF480表达独立与显著较差的患者存活率相关.
在SHG-44细胞中,zNF480的表达被ademetionine disulfate tosylate抑制.
ZNF480涉及多种与瘤相关的信号通路,包括Notch信号.
在LGG微环境中,ZNF480促进高免疫状态,增强免疫细胞透 (例如M2巨细胞).
ZNF480与PD-L1.1等免疫检查点呈正相关性.

结论:

这项研究确立了ZNF480作为LGG的新型,独立的预后因素,与患者不良结果相关.
ZNF480通过促进免疫细胞透和免疫检查点表达来影响LGG瘤微环境.
向ZNF480可能为分子向疗法或LGG的联合免疫疗法提供了一个有希望的策略.

关键词:

免疫微环境是一个微环境.较低级别的结质瘤预测预后预测预测 ZNF48080 是一个

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