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一个编码蛋白质的基因表达图谱,来自怀孕和非怀孕山羊的大脑

María Gracia Luigi-Sierra¹, Dailu Guan¹, Manel López-Béjar²

¹Centre for Research in Agricultural Genomics (CRAG), CSIC-IRTA-UAB-UB, Bellaterra, Spain.

Frontiers in genetics

|July 31, 2023

概括

这项研究绘制了山羊大脑基因表达的地图,揭示了与前后轴相关的区域模式. 怀孕显著改变嗅觉球泡基因表达,影响行为.

科学领域:

神经科学是一个神经科学.
基因组学就是基因组学.
动物科学动物科学

背景情况:

大脑的复杂性需要详细的地图书来理解功能.
羊脑的基因表达模式在很大程度上没有特征.
研究12个大脑区域的基因表达,为大脑组织提供了关键的见解.

研究的目的:

为山羊大脑构建一个全面的蛋白质编码基因表达图谱.
分析怀孕和非怀孕山羊之间大脑区域的转录组差异.
为了将基因表达模式与解剖位置和生理状态相关联.

主要方法:

来自七只穆尔西亚诺-格拉纳迪纳山羊的12个不同的大脑区域的RNA测序.
生物信息分析包括主要组件分析和等级聚类.
不同基因表达分析比较怀孕和非怀孕的个体.

主要成果:

在山羊大脑区域表达了14889到15592个蛋白质编码基因.
基因表达模式通常与前后轴相关,小脑和腺组织除外.
怀孕诱导了嗅觉球泡基因表达的显著变化,与嗅觉功能和行为有关.

结论:

羊脑mRNA表达模式在很大程度上沿前后轴组织,有显著的例外.
孕期对嗅觉球泡基因表达有深远的影响,这表明它在孕产妇的行为中发挥了作用.
这本地图集为山羊神经生物学和比较基因组学提供了基础资源.

关键词:

在RNA-Seqq.不同的基因表达 (DGE)一个胚胎囊泡.脑区 (encephalon) 是一个脑区.怀孕的时间怀孕的时间一只山羊,一只山羊.

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