来自人类胎盘的细菌细胞外囊泡的微生物DNA的放大
Ramkumar Menon1, Kamil Khanipov2, Enkhtuya Radnaa1
1Division of Basic Science and Translational Research, Department of Obstetrics and Gynecology, The University of Texas Medical Branch at Galveston, Galveston, TX, United States.
Frontiers in microbiology
|July 31, 2023
概括
细菌细胞外囊泡 (BEVs) 存在于胎盘中,可以解释在研究中检测到的微生物DNA. 这些囊泡不太可能引起显著的免疫反应或不良妊娠结果.
科学领域:
- 微生物学 微生物学
- 免疫学 免疫学 免疫学
- 产科 产科 产科 产科 产科
背景情况:
- 胎盘在胎儿发育中的作用至关重要.
- 胎盘微生物组的存在受到争论.
- 细菌细胞外囊泡 (BEVs) 被研究为微生物DNA的来源.
研究的目的:
- 测试来自格拉姆阴性细菌的细菌细胞外囊泡 (BEV) 是否是胎盘中微生物DNA的来源.
- 开发一种协议,以从人类胎盘组织中分离和鉴定BEV.
主要方法:
- 从孕期胎盘组织中分离的BEVs.
- 使用尺寸,LPS和ompA含量以及西方斑点来描述BEV.
- 分析了BEV和胎盘DNA的分类学和多样性概况.
- 研究了母体和胎盘细胞对BEV的吸收.
- 评估了BEVs的炎症性细胞因子生产.
主要成果:
- 隔离的BEVs (130-140nm) 含有LPS和 ompA.
- BEV显示微生物DNA序列读数高于胎盘样本.
- 在BEVs和胎盘之间的微生物组中存在显著的重叠.
- BEV被母体和胎盘细胞内化.
- 高剂量的BEVs在巨细胞中诱导了细胞因子的产生,但不是胎盘细胞.
结论:
- BEVs是怀孕的正常组成部分,可能通过血液传播到达胎盘.
- BEVs可能会引起轻度炎症,但不太可能损害胎儿发育.
- 由于它们的存在和DNA含量,BEVs可以混胎盘微生物组研究.
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