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相关概念视频

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
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Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
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相关实验视频

Updated: Jul 20, 2025

Identification of Functionally-Relevant Lentivirus Integration Sites in an Insertional Mutagenesis Cell Library
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通过整合基因组测序和转录组数据来识别功能调节突变块.

Mingyi Yang1,2, Omer Ali3,4, Magnar Bjørås1,5

  • 1Department of Microbiology, Oslo University Hospital and University of Oslo, Oslo, Norway.

iScience
|July 31, 2023
PubMed
概括

鉴定与疾病相关的功能单核酸变体 (SNV) 是很困难的. 我们的工具BayesPI-BAR版本3 (bpb3) 使用基因组和转录基因组数据发现功能突变块 (FMB),以揭示疾病机制.

关键词:
生物计算方法是一种生物计算方法.生物信息学是一种生物信息学.生物科学 生物科学生物科学工具 生物科学工具俄米克斯 (Omics) 是一个电子游戏.

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科学领域:

  • 基因组学就是基因组学.
  • 生物信息学是一种生物信息学.
  • 分子生物学分子生物学

背景情况:

  • 数以百万计的单核酸变体 (SNV) 在人类基因组中存在.
  • 鉴定与疾病相关的功能性SNV仍然是遗传研究中的一个重大挑战.

研究的目的:

  • 推出BayesPI-BAR版本3 (bpb3),这是一个用于分析非编码SNV的新工具.
  • 通过整合基因组测序和转录组数据来识别功能突变块 (FMB).

主要方法:

  • 实施了两级贝叶斯方法,并采用了蛋白质-DNA相互作用的生物物理模型.
  • 计算转录因子 (TF) -DNA结合亲和力变化使用来自1700多个TF动机的聚类位置重量矩阵 (PWM).
  • 综合表观遗传数据,如DNA甲基组,用于增强FMB扫描.

主要成果:

  • 鉴定了由高频SNVs和差异表达基因调控区域附近的TF结合亲和力显著变化的FMBs.
  • 使用毛囊淋巴瘤和黑色素瘤的数据集,证明了强大而自动的FMB识别.
  • 在经过测试的数据集中,bpb3成功识别了FMB.

结论:

  • 通过分析基因组和转录基因组数据,bpb3提供了对疾病病理机制的宝贵见解.
  • 该工具有助于识别疾病的潜在治疗点.
  • bpb3提供了一种功能性SNV分析的可靠方法.