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通过降低LHCGR/PGR通路的调节,孕激素原始卵巢刺激 (PPOS) 的延迟排卵

Yating Xie¹, Wenya Guo¹, Xi Shen¹

¹Department of Assisted Reproduction, Shanghai Ninth People's Hospital, Shanghai JiaoTong University School of Medicine, Shanghai, P.R. China.

|July 31, 2023

概括

孕激素主导的卵巢刺激 (PPOS) 通过抑制黄素激素 (LH) 和孕激素来延迟排卵. 这影响了关键的排卵基因,揭示了LHCGR-PGR通路.

科学领域:

生殖内分泌学生殖内分泌学
卵巢生理学卵巢生理学
分子生物学分子生物学

背景情况:

孕激素主导的卵巢刺激 (PPOS) 是一种新兴的排卵诱导协议.
控制PPOS诱导排卵的精确机制及其调节途径仍然不完全理解.

研究的目的:

在PPOS协议中阐明导致排卵延迟的调节机制.
研究激素波动和基因表达在PPOS介导的排卵时间中的作用.

主要方法:

在小鼠模型中模拟了临床PPOS协议.
量化排卵卵细胞,雌激素,孕和素激素 (LH) 的水平在触发后.
利用mRNA提取,实时PCR,血素和欧染色以及免疫光学来分析卵巢组织和信号通路.

主要成果:

治疗PPOS导致排卵显著延迟,在触发后12.5小时观察到.
抑制的LH水平前触发减少了在排卵前毛囊上的素化激素/胆激素受体 (LHCGR) 表达.
降低了孕激素合成和血液水平,以及降低了孕激素受体 (PGR) 表达,在触发后观察到.
通过PGR调节的关键排卵基因 (ADAMTS-1,VEGF-A,EDN2) 被下调,导致排卵延迟.

结论:

在排卵诱导之前,PPOS抑制LH水平,并在激发后降低孕激素合成.
LHCGR-PGR通路在PPOS介导的排卵时间中具有关键作用.
在PPOS协议中,LHCGR-PGR通路的下调最终会延迟排卵.

关键词:

内分泌系统生理学内分泌系统生理学分子生物学分子生物学

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