糖尿病病患者的尿路微生物群和血清代谢物分析
Yan Yang1, Chiyu Ma2, Shishi Li1
1Department of Nephrology and Blood Purification, The First Affiliated Hospital of Jinan University, Jinan University, Guangzhou, 510632, China.
Heliyon
|July 31, 2023
概括
糖尿病病 (DKD) 与明显的尿路微生物群变化有关,蛋白质细菌和酸性细菌的增加. 素代谢的改变可能会推动DKD的进展,提供新的诊断和治疗点.
科学领域:
- 微生物学 微生物学
- 腎臟病學 (nephrology) 是一種醫學專業.
- 代谢学 代谢学 代谢学
背景情况:
- 糖尿病病 (DKD) 是糖尿病的严重并发症,可能导致慢性功能衰竭.
- 新出现的证据表明,泌尿道含有居住的微生物群,挑战了无菌尿的概念.
- 尿路微生物群在DKD病变发生过程中的确切作用仍然在很大程度上是未知的.
研究的目的:
- 鉴定DKD患者尿道微生物群的特征.
- 探索尿路微生物群和DKD中的血清代谢物之间的关系.
- 确定DKD的潜在诊断和治疗点.
主要方法:
- 16S rRNA基因测序用于分析19名DKD患者和15名健康对照者的尿液微生物群组成.
- 血清代谢物概况使用液态染色学-并联质谱法 (LC-MS/MS) 进行评估.
- 研究了临床数据,尿液微生物群和血清代谢物之间的相关性.
主要成果:
- 与健康对照组相比,DKD患者表现出明显的尿路微生物群概况,阿尔法多样性减少.
- 类蛋白质细菌和酸性细菌在DKD中富含,而Firmicutes和Bacteroidetes则耗尽.
- 观察到短链脂肪酸 (SCFA) 和与蛋白质结合的尿素毒素 (PBUTs) 的特定变化,与氨酸和氨酸代谢有关.
结论:
- 该研究确立了尿路微生物群和DKD中的血清代谢物之间的功能联系.
- 蛋白质细菌和酸性细菌被确定为DKD中尿路微生物群的关键组成部分.
- 氨酸代谢的改变被认为是DKD进展的重要因素.
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