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小辅助UpRNA 56 (SAUR56) 调节了大米中的标题日期

Zhe Zhao¹, Tengkui Chen¹, Jicheng Yue¹

¹National Plant Space Breeding Engineering Technology Research Center, South China Agricultural University, Guangzhou, 510642 People's Republic of China.

Molecular breeding : new strategies in plant improvement

|July 31, 2023

概括

小辅助RNA (SAUR) 基因OsSAUR56对于调节大米的关键作用. 它在花期控制中的功能为米育种中的基因工程提供了潜力.

科学领域:

植物遗传学和分子生物学
农学和作物科学农学和作物科学

背景情况:

标题日期是影响大米产量的关键农学特征.
小辅助RNA (SAUR) 基因在标题日期调节中的作用在很大程度上是未知的.

研究的目的:

为了研究大米中SAUR基因的生物功能.
阐明OsSAUR56在调节标题日期和开花时间方面的特定作用.

主要方法:

使用CRISPR-Cas9基因编辑来创建SAUR基因的功能丧失突变.
对OsSAUR56.5进行表达分析和蛋白质定位研究.
进行了 Haplotype-phenotype 关联分析,以评估 OsSAUR56 在化中的作用.

主要成果:

在不同的光周期条件下,OsSAUR56的功能丧失显著影响了航线日期.
OsSAUR56主要表达在中,其蛋白质存在于细胞质和细胞核中.
OsSAUR56通过调节关键基因OsGI,Ghd7和DTH8.8的表达来调节开花时间.

结论:

OsSAUR56在控制大米标题日期和开花时间方面发挥着至关重要的作用.
OsSAUR56与标题日期的关联表明它在养大米期间的重要性.
OsSAUR56是基因工程的一个有希望的目标,以改善米育种中的开花时间.

关键词:

标题日期标题日期自然变化的自然变化.大米大米大米大米大米萨尔家族 (SAUR) 是一个家族.

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