希斯脱酶9在阴影下促进阴囊细胞特异性辅酶反应
Nguyen Hoai Nguyen1, Benny Jian Rong Sng1,2, Hui Jun Chin1
1Temasek Life Sciences Laboratory, National University of Singapore, 1 Research Link, Singapore, 117604, Singapore.
The Plant journal : for cell and molecular biology
|July 31, 2023
概括
在HISTONE DEACETYLASE 9 (HDA9) 中的功能丧失突变通过损害auxin-responsive基因激活来特别降低Arabidopsis中阴影诱导的阴囊延长. HDA9与PIF7相互作用,调节避免阴影的反应.
科学领域:
- 植物生物学 植物生物学
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
背景情况:
- 避阴综合症 (SAS) 引发了植物形态变化,如皮细胞延长.
- 希斯脱酶9 (HDA9) 是植物中一个关键的表观遗传调节剂.
研究的目的:
- 为了研究HDA9在调节Arabidopsis中阴影诱导的阴囊延长中的作用.
- 阐明HDA9控制SAS的分子机制.
主要方法:
- 阿拉比多普西斯塔利亚纳基因突变分析 (hda9).
- 特定器官的转录组分析.
- 染色体免疫沉 (ChIP) 试验.
- 蛋白质与蛋白质相互作用研究.
主要成果:
- hda9突变者表现出减弱的阴影诱导的阴囊延长,但没有其他SAS特征.
- HDA9调节素反应基因 (SAURs,AUX/IAAs) 的阴影诱导表达.
- 在物理上,HDA9与植物染色体交互因子7 (PIF7) 相互作用,并与基因位置结合.
结论:
- 在避免阴影期间,HDA9在阴囊延长中发挥着特定的作用.
- HDA9作为转录协调剂,可能被PIF7招募,以激活 hypocotyls 中的辅酶信号传递.
- 这项研究揭示了HDA9是控制阴影反应的皮细胞特定机制的关键组成部分.
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