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环-DTL海绵miR-758-3p通过调节DCUN1D1表达来加速宫癌恶性进展
Xiaoning Luo1, Jiewen Liu1, Xiangcai Wang1
1Department of Oncology, The First Affiliated Hospital of Gannan Medical University, Gannan Medical University, Ganzhou, Jiangxi, China.
Journal of biochemical and molecular toxicology
|July 31, 2023
概括
循环RNA DTl (circ-DTL) 通过海绵miR-758-3p和调节DCUN1D1.1,促进子宫癌 (CC) 的进展. 击败circ-DTL会在体内抑制CC细胞的生长,迁移,入侵和瘤形成.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
背景情况:
- 循环RNAs (circRNAs) 越来越多地被认为是癌症进展中的角色.
- 在宫癌 (CC) 中,无环牙的E3无素蛋白质酶同类素 (circ-DTL) 的特异性功能和临床意义在很大程度上仍未得到研究.
研究的目的:
- 阐明circ-DTL在宫癌的发展和进展中的作用和潜在机制.
- 在CC中调查涉及circ-DTL,miR-758-3p和DCUN1D1的监管途径.
主要方法:
- 定量实时聚合酶链反应 (qRT-PCR) 用于基因表达分析.
- 细胞增殖,细胞循环,细胞亡,迁移和入侵试验 (CCK-8,EDU,流细胞计,伤口愈合,transwell).
- 双 luciferase 记者和 RNA 免疫沉降 (RIP) 测试以确定分子相互作用;异种移植小鼠模型用于体内验证.
主要成果:
- 在CC组织和细胞中,Circ-DTL和DCUN1D1显著上调,而miR-758-3p则下调.
- 抑制circ-DTL抑制了CC细胞的增殖,迁移,入侵和瘤生长,同时诱导细胞循环停止和亡.
- 发现Circ-DTL对miR-758-3p进行了海绵作用,miR-758-3p通过抑制DCUN1D1.1来抑制CC进展.
结论:
- 在宫癌中,Circ-DTL作为瘤促进剂起作用.
- 该circ-DTL/miR-758-3p/DCUN1D1轴在调节CC的发展和进展方面发挥着至关重要的作用.
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