在口腔状细胞癌患者中,miR-99a-5p作为淋巴结转移预测的生物标志物
Cristiane Pereira Vargas Cousseau1, Bruna Pereira Sorroche1, Renan de Jesus Teixeira1
1Molecular Oncology Research Center, Barretos Cancer Hospital, Barretos, Brazil.
Head & neck
|July 31, 2023
概括
研究人员在口腔状细胞癌 (OSCC) 瘤中确定了特定的微RNA (miRNA). 这些miRNA可以帮助预测早期OSCC患者的淋巴结转移,从而改善治疗决策.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 基因组学就是基因组学.
背景情况:
- 转移性淋巴结的参与对于口腔状细胞癌 (OSCC) 的预后和治疗至关重要.
- 许多患有临床阴性淋巴结 (cN0) 的早期OSCC患者有隐性转移 (pN+).
- 精确的淋巴结分期对于有效的OSCC管理至关重要.
研究的目的:
- 在初级OSCC瘤中识别差异表达的微RNA (miRNA).
- 在cN0OSCC患者中区分病理阳性 (pN+) 和阴性 (pN0) 淋巴结.
- 为了发现早期淋巴结转移检测的潜在miRNA生物标志物.
主要方法:
- 在20名OSCC患者 (10pN+,10pN0) 中使用纳米链nCounter平台对798个miRNAs进行微RNA表达概况分析.
- 通过RT-qPCR在39名OSCC患者 (15pN+,24pN0) 的独立队列中验证差异表达的miRNAs.
主要成果:
- 八个miRNAs在pN0和pN+OSCC患者组之间显示了差异性表达.
- hsa-miR-99a-5p被确定为一个显著的差异化因素.
- hsa-miR-99a-5p在预测淋巴结转移风险方面表现出高灵敏度和特异性.
结论:
- hsa-miR-99a-5p是一种有前途的生物标志物,用于检测初级OSCC中淋巴结转移.
- 这种miRNA可以帮助识别需要进一步干预的高风险患者.
- 在OSCC中改善非侵入性分期和个性化治疗的潜力.
相关概念视频
MicroRNAs
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...
lncRNA - Long Non-coding RNAs
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA (lncRNA)...
MicroRNAs
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...


