足部感染的临床微生物学概况:一个前性,横截面研究
Swathi Holla1, Sevitha Bhat1, Archana Bhat1
1Department of Microbiology, Kasturba Medical College, Mangalore, Manipal Academy of Higher Education, Manipal, India.
Wounds : a compendium of clinical research and practice
|July 31, 2023
概括
在足部感染中,生物膜检测至关重要. 观察到生物膜的产生率很高,特别是Pseudomonas aeruginosa,与多药性耐药性 (MDR) 和甲素耐药黄金葡萄球菌 (MRSA) 相相关.
科学领域:
- 微生物学 微生物学
- 传染性疾病 传染性疾病
- 足部医学是一门专业.
背景情况:
- 足部感染在糖尿病患者和非糖尿病患者中普遍存在.
- 生物膜的形成使感染管理和治疗结果复杂化.
- 早期检测生物膜对于有效的预防策略至关重要.
研究的目的:
- 调查足部感染的临床微生物学概况.
- 为了确定在孤立的微生物中生物膜产生的流行率.
- 评估生物膜形成与抗菌素耐药性之间的关联.
主要方法:
- 临床分离物的微生物学识别和抗生素敏感性测试.
- 使用微板方法对生物膜生产进行选.
- 多重PCR用于检测黄金葡萄球菌中的特定基因 (ica,cna,hlg).
主要成果:
- 在117名患者中观察到60.6%的培养阳性率.
- 格拉姆阴性细菌占主导地位 (73.3%),金黄色葡萄球菌是最常见的分离物 (26.7%).
- 总体生物膜生产率为54.2%,其中Pseudomonas aeruginosa是最常见的生产者 (82.8%).
- 生物膜形成与多药性耐药性 (MDR),甲素耐药黄金葡萄球菌 (MRSA) 和之前广泛的抗生素治疗有显著的关联.
结论:
- 在糖尿病足部感染中,MRSA或MDR菌株的隔离可能表明由于生物膜而导致的潜在治疗失败.
- 早期检测产生生物膜的生物体对于预防慢性感染至关重要.
- 在确定生物膜时,建议立即除和清洁伤口.
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