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野生北美 (Anas platyrhynchos) 的染色体水平参考基因组

Philip Lavretsky¹, Flor Hernández¹, Thomas Swale²

¹Department of Biological Sciences, University of Texas at El Paso, El Paso, TX 79968, USA.

G3 (Bethesda, Md.)

|July 31, 2023

概括

这项研究介绍了首个高质量的野生 (Anas platyrhynchos) 的基因组组合. 这种资源揭示了与国内鸟基因组相比显著的差异,影响了我们对化和进化的理解.

科学领域:

基因组学就是基因组学.
进化生物学进化生物学
鸟类学鸟类学是一门学科.

背景情况:

鸟 (Anas platyrhynchos) 是全球重要的鸟类,在历史上对食物和养非常重要.
现有的马拉德基因组组件主要基于国内或遗传不确定的样本.
野生的高质量参考基因组对于理解它们的进化历史和化影响至关重要.

研究的目的:

报告首次高质量的基因组组装和注释来自北美的经过基因验证的野生马拉德.
提供一个强大的基因组资源,用于研究鸟进化,物种化和化.
为了比较野生鸟基因组与现有的国内/未知遗产组合,以揭示遗传差异.

主要方法:

基因组组装使用枪,芝加哥和 Dovetail Hi-C 库.
基因预测和基于相似性的注释方法,包括基因本体学 (GO) 术语关联.
通过将以前发表的野基因组与新野生野组合对齐进行比较分析.

主要成果:

一个高质量的基因组组 (NAwild_v1.0) 大约1.04 Gb,其中98.3%在染色体水平的支架中.
23,584个功能基因的注释,其中19242个与GO术语相关,达到95.4%的BUSCO完整性.
证明先前发表的鸟基因组表现出更高的同卵性和更低的核酸多样性,导致与野生鸟组合相比有偏见的人口统计推断.

结论:

NAwild_v1.0组件提供了一个重要的资源,帮助我们理解鸟基因组的进化,物种化和适应.
这种野生草基因组有助于识别在化过程中显著改变的基因.
这些发现强调了使用经过基因审查的野生样本来进行准确的基因组研究和进化推断的重要性.

关键词:

安纳斯的平面鱼 (platyrhynchos) 是一个鱼.染色体尺度上的染色体尺度基因组组装组合的基因组.野生马拉德 (Wild Mallard) 是一个野生动物.

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