相关实验视频
Updated: Jul 20, 2025

06:10
Following Cell-fate in E. coli After Infection by Phage Lambda
Published on: October 14, 2011
23.5K
对Clostridium perfringens菌体endolysin cpp-lys的表征及其对生菜的应用
Xiaonan Zhao1, Lulu Li1, Qing Zhang1
1Shandong Key Laboratory of Animal Disease Control and Breeding, Institute of Animal Science and Veterinary Medicine, Shandong Academy of Agricultural Sciences, PR China; Key Laboratory of Livestock and Poultry Multi-omics of MARA, PR China.
International journal of food microbiology
|July 31, 2023
概括
这项研究表明,endolysin cpp-lys有效地控制了沙拉上的食品传播病原体Clostridium perfringens. 该蛋白质稳定且无毒,为食品安全提供了有前途的解决方案.
科学领域:
- 微生物学 微生物学
- 食品科学 食品科学 食品科学
- 生物技术是生物技术.
背景情况:
- 杆菌是重要的食物传播病原体,造成严重的健康和经济影响.
- 控制食品中的C. perfringens污染仍然是公共卫生和食品工业面临的关键挑战.
研究的目的:
- 克隆,过度表达,并描述来自C. perfringens菌体cpp.lys的内素cpp-lys.
- 评估内素cpp-lys在控制沙拉上的C. perfringens污染的疗效.
- 评估Endolysin cpp-lys对潜在的食品工业应用的稳定性和安全性.
主要方法:
- 基因克隆和cpp-lys的蛋白质过度表达.
- 恩多利辛cpp-lys活性,稳定性 (温度,pH) 和储存效应的表征.
- 评估cpp-lys对C. perfringens J1在生菜上的有效性.
- 评估cpp-lys对真核细胞的细胞毒性以及金属离子 (Zn2+) 和EDTA的影响.
主要成果:
- 恩多利辛cpp-lys对七种C. perfringens菌株表现出溶解活性.
- 蛋白质在广泛的温度范围 (4-50°C) 和pH值 (4-9) 中保持稳定,在各种温度下 (除了37°C和42°C外) 储存30天后没有活动损失.
- 在生菜上,cpp-lys在15分钟内显著降低了C. perfringens J1>4-log,并且对真核细胞没有细胞毒性. Zn2+ 抑制了活性,而 EDTA 治疗降低了疗效.
结论:
- 恩多利辛cpp-lys是一种强大而稳定的抗克洛斯特菌的药物.
- 它的非细胞毒性和对食品表面的有效性表明食品安全应用的巨大潜力.
- 对优化cpp-lys应用的进一步研究,可能考虑其与金属离子的相互作用,是有必要的.
相关概念视频
Lysogenic Cycle of Bacteriophages
62.4K
In contrast to the lytic cycle, phages infecting bacteria via the lysogenic cycle do not immediately kill their host cell. Instead, they combine their genome with the host genome, allowing the bacteria to replicate the phage DNA along with the bacterial genome. The incorporated copy of the phage genome is called the prophage. Some prophages can re-activate and enter the lytic cycle. This often occurs in response to a perturbation, such as DNA damage, but can also transpire in the absence of...
62.4K
Viral Replication: Lytic Cycle
73
Bacteriophages, or phages, are viruses that specifically infect bacteria. Among them, T-even bacteriophages, such as T4, exhibit a well-characterized lytic replication cycle in Escherichia coli (E. coli). This process ensures the rapid proliferation of the virus while ultimately leading to the destruction of the bacterial host.Attachment and DNA InjectionThe infection process begins with the recognition and binding of the T4 phage to the E. coli cell surface. Tail fibers of the phage...
73
Viral Replication: Lysogenic Cycle
76
The lysogenic cycle is a crucial viral replication strategy that allows bacteriophages to persist within host cells without immediately destroying them. This process is primarily observed in temperate phages, such as bacteriophage lambda (λ), which infects Escherichia coli. The cycle allows the viral genome to persist across bacterial generations while keeping host cells viable.Integration of the Viral GenomeUpon infection, bacteriophage lambda attaches to the bacterial surface and injects...
76
Lytic Cycle of Bacteriophages
70.9K
Bacteriophages, also known as phages, are specialized viruses that infect bacteria. A key characteristic of phages is their distinctive “head-tail” morphology. A phage begins the infection process (i.e., lytic cycle) by attaching to the outside of a bacterial cell. Attachment is accomplished via proteins in the phage tail that bind to specific receptor proteins on the outer surface of the bacterium. The tail injects the phage’s DNA genome into the bacterial cytoplasm. In the...
70.9K

