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破译Staphylococcus xylosus和Staphylococcus equorum在干燥固化火腿模型系统中对Penicillium nordicum的作用模式

Eva Cebrián¹, Elia Roncero¹, Josué Delgado¹

¹Higiene y Seguridad Alimentaria, Instituto de Investigación de Carne y Productos Cárnicos (IProCar), Facultad de Veterinaria, Universidad de Extremadura, Avda. de las Ciencias s/n, 10003 Cáceres, Spain.

International journal of food microbiology

|July 31, 2023

概括

两种葡萄球菌菌株抑制了Penicillium nordicum的生长和干燥化火腿中 ochratoxin A (OTA) 的产生. 它们的抗真菌作用在15°C时更强,这与营养竞争和真菌代谢改变有关.

科学领域:

食品微生物学食品微生物学
菌类学菌类学是指菌类学.
生物技术是生物技术.

背景情况:

北半球是一种关键的 ochratoxin A (OTA) 生产者,在干的火腿中.
葡萄球菌 (Staphylococcus xylosus) 和葡萄球菌 (S. equorum) 是对OTA污染的潜在生物控制剂 (BCA).
这些BCA对P. nordicum的抗真菌机制尚不清楚.

研究的目的:

为了阐明S. xylosus和S. equorum对P. nordicum的作用模式,在一个干燥固化的火腿模型中.
研究温度对BCA疗效和真菌-细菌相互作用的影响.

主要方法:

使用活细胞/死细胞和无细胞的共同培养试验.
营养利用,利基重叠指数 (NOI) 和双培养试验.
对挥发性化合物,OTA降解和真菌蛋白质组的分析.

主要成果:

活体葡萄球菌在15°C和20°C时抑制了P. nordicum的生长,在15°C时更有效.
营养主导地位随温度变化;BCA在15°C时占主导地位,P. nordicum在20°C时占主导地位.
反对作用涉及营养竞争,改变细胞壁完整性和抑制OTA生物合成,而不是挥发性化合物或OTA降解.

结论:

S. xylosus和S. equorum对P. nordicum的对抗作用取决于温度,有利于较低的温度 (15°C).
机制包括对营养和空间的竞争,影响真菌细胞壁的完整性和OTA生产途径.
这些发现支持将这些葡萄球菌作为BCA用于控制干燥硬化火腿中的OTA.

关键词:

生物控制生物控制碳利用模式的使用模式竞争力竞争力竞争力干燥制的火腿.奥克拉托克辛A是一种蛋白质组学是指蛋白质组学.

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