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益生菌银河糖的酶合成从银河糖从阿拉伯衍生的银河糖

Ke Wang¹, Yihong Xu², Zehui Xuan¹

¹School of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.

|July 31, 2023

概括

一种新的酶法有效地从植物衍生的银河糖中合成银河糖类 (GOS). 这种可持续的工艺实现了高产量,并利用可回收的酵母细胞用于GOS生产.

科学领域:

生物技术是生物技术.
酶学是一种酶学.
碳水化合物化学碳水化合物化学

背景情况:

галактоолигосахарид (GOS) 是一种有价值的益生菌.
目前的GOS合成方法可能是低效的或产生副产品.
来自植物的银河糖提供了一个可持续的基质来源.

研究的目的:

开发一种使用植物衍生的银河糖合成GOS的新型酶过程.
优化GOS生产条件并对产生的产品进行表征.
评估酶源的可重复使用性.

主要方法:

查酵母乳糖菌 (Kluyveromyces lactis) 的β-银酸酶活性.
使用单因子和响应表面方法来优化GOS合成条件.
使用质谱法 (MS) 和核磁共振 (NMR) 谱的GOS结构的表征.
在多个合成批次中对酶细胞可重复使用性的评估.

主要成果:

从银河糖中合成GOS的新型酶过程被建立起来.
在优化条件下,GOS最高收益率为45%.
确定的主要GOS结构包括Gal-β-D-(1→6) -Gal,Gal-β-D-(1→3) -Gal,以及Gal-β-D-(1→6) -Gal-β-D-(1→6) -Gal.
含有β-银酸酶的酵母细胞至少回收了30批次,酶活性保留超过60%.

结论:

成功开发了一种可持续和高效的酶法,用于从植物衍生的银河糖中生产GOS.
优化的工艺产生高纯度的GOS,副产品易于分离.
使用的酵母细胞显示出出色的可重复使用性,强调了该过程的经济可行性和环保性.

关键词:

批量合成批量合成银河氧糖糖类 (Galactooligosaccharides) 是一种有氧糖类.银河糖 (Galactose) 是一种银河糖.阿拉伯口香糖 (Gum arabic) 是一种阿拉伯口香糖.这就是β-Galactosidase.

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