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在小麦-甜轮系统中长期化肥和甜种植会通过调节土壤phoD - - 寄存细菌群落来降低性胺酶活性

Lei Wang¹, Hui Zhang², Cong Xu²

¹National Agricultural Experimental Station for Agricultural Environment, Luhe, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.

The Science of the total environment

|July 31, 2023

概括

长期的化肥会通过降低土壤pH而损害土壤循环细菌. 结合有机和无机肥料可以减轻这些对性基化酶 (ALP) 社区的负面影响.

科学领域:

土壤微生物学土壤微生物学
生物地质化学循环生物地质化学循环
农业科学农业科学

背景情况:

含有性单酶 (ALP) 的细菌 (含有phoD的社区) 对于将有机转化为可用的至关重要.
了解如何施肥和作物类型影响这些社区在作物轮换是至关重要的,但不太了解.
在小麦-甜轮换的九年实地实验中,研究了这些影响.

研究的目的:

分析phoD携带细菌群落对不同施肥策略和作物类型的反应.
调查这些社区,它们的功能活动 (ALP) 和土壤物理化学性质之间的相关性.
为了阐明小麦 - 甜旋转系统中的相互作用.

主要方法:

使用四种施肥策略进行实地实验:没有施肥 (CK),无机NK,无机NPK和NPK+有机肥 (NPKM).
在作物收获后抽取土壤样本进行分析.
细菌社区组成的高通量测序和ALP活动的酶学.
结构方程建模用于评估变量之间的关系.

主要成果:

长期 (N) 施肥,特别是无机N,降低了土壤pH和ALP活性,同时增加了可用的 (AP).
可用的 (AP) 含量在甜季节比小麦季节更高.
无机N受精显著改变了phoD的细菌群落,减少了多样性和关键品种如Acuticoccus,Methylibium,Rhizobacter和Roseivivax的丰富性.
土壤pH和AP被确定为phoD寄存细菌群落和ALP活动的关键调节者.

结论:

施肥策略和作物类型显著影响D寄存细菌群落及其循环功能.
由于无机肥的土壤酸化对这些关键的细菌群体产生不利影响.
无机和有机肥料的联合应用可以有效地减轻肥对土壤P循环细菌的不良影响.

关键词:

性性基基化酶.长期的实地实验长期的实地实验化肥的受精方式土壤的pH值是什么 phoD - 庇护社区.

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