来自Anoxybacillus vranjensis ST4的高度稳定和多功能α-氨酶适合各种应用
Marinela Šokarda Slavić1, Milan Kojić2, Aleksandra Margetić1
1University of Belgrade, Institute of Chemistry, Technology and Metallurgy, National Institute of the Republic of Serbia, Department of Chemistry, Belgrade, Republic of Serbia.
International journal of biological macromolecules
|July 31, 2023
概括
一种来自Anoxybacillus vranjensis ST4 (AVA) 的新型α-氨酶被成功表达和净化. 这种热稳定酶有效地化各种原始粉,显示出工业潜力.
科学领域:
- 生物化学 生物化学
- 酶学 是一种酶学.
- 分子生物学分子生物学
背景情况:
- 酶是重要的生物催化剂.
- 热友性酶在工业过程中提供稳定性优势.
- 阿尔法氨基酶是粉水解的关键.
研究的目的:
- 为了克隆,表达和净化来自Anoxybacillus vranjensis ST4 (AVA) 的α-氨酶.
- 描述AVA的生物化学特性和粉水解能力.
- 评估AVA的工业适用性.
主要方法:
- 基因克隆进入pMALc5HisEk载体.
- 从大肠杆菌 (ER2523) 进行表达和净化.
- 在不同温度和pH值下进行酶活性测定.
- 确定粉水解效率的方法.
主要成果:
- AVA是一种66 kDa的单体,属于GH13_5亚家族,具有CBM20粉结合域.
- 在60-80°C处具有最佳活性,在pH值4.0-9.0之间具有稳定性.
- 生玉米,小麦,卜和土豆粉的高效水解,产量高的麦芽糖,麦芽糖和麦芽糖.
结论:
- AVA表现出高表达水平和显著的热稳定性和pH稳定性.
- 其高效的原始粉水解和产品形象使其成为工业应用的多功能候选者.
- 该酶的特性表明,在食品加工和生物燃料生产等行业具有重大潜力.
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