在磁性纳米颗粒中的固定性乙胆酶用于使用毛细管电泳系统进行线内抑制研究
Diogo Alexandre Siebert1, Natália Bruzamarello Caon2, Michele Debiasi Alberton3
1Laboratório de Eletroforese Capilar, Departamento de Química, Universidade Federal de Santa Catarina, Trindade, CEP 88040-900, Florianópolis, SC, Brazil.
Analytica chimica acta
|July 31, 2023
概括
这项研究介绍了一种基于磁纳米粒子的新型酶微反应器,用于乙胆化酶试验. 开发的方法在分析植物提取物的酶抑制方面表现出稳定性和有效性.
科学领域:
- 分析化学 分析化学
- 生物化学 生化学
- 材料科学 材料科学 材料科学
背景情况:
- 酶试验对于生物化学分析和药物发现至关重要.
- 微型酶试验在样品和试剂消耗方面具有优势.
- 乙胆酶 (AChE) 是各种应用的关键酶标,包括农药和药物开发.
研究的目的:
- 开发和描述一种用于乙胆酶 (AChE) 试验的新型酶微反应器.
- 使用奇托和甲将ACHE固定在磁性纳米粒子 (MnFe2O4) 上.
- 为了评估微反应器的性能,稳定性和应用,分析植物提取物中的酶抑制.
主要方法:
- 在MnFe2O4磁纳米粒子上进行酶固定.
- 纳米粒子在毛细血管中的交叉链接使用奇托和甲.
- 使用磁铁组装微反应器.
- 酶动力学研究 (Km确定) 和抑制剂测定 (neostigmine的IC50).
- 适用于七种植物物种的醇提取物.
主要成果:
- 固定化的酶微反应器表现出良好的稳定性,支持至少25个实验.
- 迈克利斯常数 (Km) 确定为1.12 mmol L-1,可与文献值进行比较.
- 评估了neostigmine的IC50,在三个微反应器中平均为29.42±3.88μmol L-1.
- 该方法成功地用于分析植物提取物中的酶抑制,Plinia cauliflora显示出显著的抑制 (42.31 ± 6.81%在100毫克L-1下).
结论:
- 新型基于磁性纳米粒子的酶微反应器为ACHE试验提供了稳定高效的平台.
- 这种方法促进了微型化的酶测定和抑制剂查.
- 该方法适用于评估像植物提取物这样的复杂矩阵中的酶抑制.
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