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Updated: Aug 1, 2026

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs
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选择性miRNA量化使用高温热凝电泳

Mario A Cornejo1, Thomas H Linz1

  • 1Department of Chemistry, Wayne State University, USA.

Analytica chimica acta
|July 31, 2023
PubMed
概括

高温热凝电泳以单核酸精度快速量化微RNA (miRNA). 这种具有成本效益的方法克服了区分类似miRNA序列的挑战,以改善疾病研究.

关键词:
凝电泳是一种凝电泳.微流体性 微流体性选择性的选择性温度 温度是指温度.这是一个小RNARNA.

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相关概念视频

Real Time RT-PCR02:57

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...