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基于键相互作用的的电膜提取

Samira Dowlatshah¹, Frederik André Hansen¹, Chen Zhou²

¹Department of Pharmacy, University of Oslo, P.O Box 1068 Blindern, 0316, Oslo, Norway.

Analytica chimica acta

|July 31, 2023

概括

这项研究引入了一种使用三酸作为无载体液体膜的酸的新型电膜提取方法. 这种方法通过结相互作用提高稳定性和选择性,避免电解问题.

科学领域:

分析化学分析化学
分离科学分离科学

背景情况:

电膜提取 (EME) 传统上使用液体膜中的离子载体进行转移.
离子载体增加了膜导电性,导致电解加速和pH不稳定.
这限制了EME用于分析的效率和可靠性.

研究的目的:

开发一种用于的无载体电膜提取方法.
研究在EME中使用有机溶剂作为纯液体膜.
在缺乏离子载体的情况下,确定负责转移的主要相互作用机制.

主要方法:

选各种有机溶剂作为液体膜用于的EME.
选择和评估三酸的提取效率和运行稳定性.
测试了16种不同的模型的提取,重点是电荷和极性特征.
从人血中提取的的分析,使用LC-MS/MS.

主要成果:

三酸证明了高质量转移和作为无载体液体膜的运行稳定性.
获得了具有净电荷为+1和极侧链有限的的选择性提取.
三酸 (受体) 和质子 (捐赠体) 之间的键被确定为关键相互作用.
从人体血中彻底提取特定 (leu-enkephalin,met-enkephalin,endomorphin) 的方法是成功的.
获得了线性校准曲线 (r2 > 0.99) 和良好的精度 (RSD < 12%).

结论:

使用三) 酸盐的无载体EME使得基于键的选择性提取成为可能.
这种方法克服了与传统EME相关的电解和pH漂移的局限性.
该方法适用于分析像血这样的复杂矩阵中的小,低极性.

关键词:

电膜提取电膜提取提取提取提取酸是一种酸.样品的准备样品的准备

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