在植物叶子中通过LA-ICP-MS进行现场元素定量成像,并与矩阵匹配的外部校准进行校准
Zewei Cui1, Man He1, Beibei Chen1
1Department of Chemistry, Wuhan University, Wuhan, 430072, China.
Analytica chimica acta
|July 31, 2023
概括
使用凝和基甲基纤维素的新型矩阵匹配基质可以通过激光切除诱导合等离子体质谱法 (LA-ICP-MS) 直接量化植物中的元素. 这种方法准确地测量了多个元素,有助于植物生物科学和环境研究.
科学领域:
- 分析化学 分析化学
- 植物科学 植物科学
- 环境科学 环境科学
背景情况:
- 对植物元素分析对生物科学,环境和毒理学研究越来越感兴趣.
- 需要能够确定植物中的元素转移,分布和含量的分析方法.
研究的目的:
- 开发植物多种元素的直接量化方法.
- 创建一个模拟的植物矩阵,使用激光除感应合等离子体质谱法 (LA-ICP-MS) 进行校准.
- 为了验证该方法的准确性和适用性,用于植物组织中的元素成像.
主要方法:
- 制备凝 (GA) 和基甲基纤维素 (HPMC) 混合物作为模拟植物基质.
- 优化GA和HPMC基质组成,包括酸度和GA:HPMC比率 (8:2).
- 应用矩阵匹配外部校准与LA-ICP-MS直接元素量化和成像.
主要成果:
- 优化的GA&HPMC基板表现出良好的元素均性和与植物认证参考材料 (CRM) 相似的信号强度.
- 实现了各种植物CRM (叶,茶,豆,) 中元素的精确量化.
- 该方法显示了17个元素的低检测极限 (0.003-104 μg g-1) 和广泛的线性范围 (0.01-10000 μg g-1).
- 在Trigonotis peduncularis叶中成功地进行了Zn,Cu,Sr和Mn的快速,现场定量元素成像.
结论:
- 开发的GA&HPMC基板可以使用LA-ICP-MS在植物中的直接元素量化.
- 这种方法为研究植物中的元素分布和转移提供了一个强大的工具.
- 该技术支持进一步探索植物系统中的元素生物可用性,运输和毒性机制.
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