特斯拉式辅助生物传感器用于基于菌体/DNA酶联合修饰的酸伊米达酸框架编码探针的双模式和双目标同时确定食源病原体
Shuai Wang1, Jianhao Hu1, Hang You1
1Key Laboratory of Advanced Mass Spectrometry and Molecular Analysis of Zhejiang Province, Institute of Mass Spectrometry, School of Material Science and Chemical Engineering, Ningbo University of Technology, Ningbo, 315200, China.
Analytica chimica acta
|July 31, 2023
概括
一个新的双模式生物传感器可以同时准确检测大肠杆菌O157:H7和沙门氏菌typhimurium. 食品安全方面的这一进步提供了敏感的,现场的病原体查,减少了假阳性.
科学领域:
- 食品安全与分析化学
- 生物传感器技术技术
- 用于检测的纳米材料
背景情况:
- 准确检测食品传播的病原体,如大肠杆菌O157:H7 (大肠杆菌) 和沙门氏菌 (S.T) 对于公共卫生至关重要.
- 现有的方法往往缺乏同时检测能力或现场适用性.
研究的目的:
- 开发一种双模式,双目标生物传感器,用于同时检测大肠杆菌和ST.
- 为了利用特斯拉来控制流量和信号独立.
- 为了实现敏感和特定的现场病原体识别.
主要方法:
- 合成的氧化伊米达酸框架 (ZIF-8) 探针与DNA酶,菌体和电活性材料 (铁/甲蓝).
- 使用特斯拉来调节流量并防止两个工作电极之间的信号干扰.
- 采用菌体特异性用于细菌捕获和DNA酶催化用于光/电化学信号生成.
主要成果:
- 实现了对大肠杆菌和ST的同时检测,具有良好的线性 (10-10^7 CFU mL−1).
- 已证明的低检测极限 (大肠杆菌为5 CFU mL-1;ST为8 CFU mL-1).
- 双模式策略有效降低了虚假阳性率.
结论:
- 开发的生物传感器能够快速,具体和敏感地在现场确定大肠杆菌和ST.
- 特斯拉集成确保了多重检测的信号完整性.
- 这个平台有望选更广泛的食物传播病原体.
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