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在慢性淋巴细胞白血病中,IL-25对恶性B细胞存活和T细胞激活的影响

Mehrnoosh Pashei¹, Farahnaz Ghahremanfard², Ehsan Manouchehri Doulabi³

¹Department of Immunology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. meh.pashaei@yahoo.com.

Iranian journal of allergy, asthma, and immunology

|July 31, 2023

概括

干白素-25 (IL-25) 通过刺激T细胞和减少瘤细胞死亡,促进慢性淋巴细胞白血病 (CLL) 细胞生存. 这表明IL-25通过IL-17RB等与炎症相关的受体在CLL发育中发挥作用.

科学领域:

免疫学免疫学免疫学
血液学血液学血液学
细胞生物学细胞生物学

背景情况:

慢性淋巴细胞白血病 (CLL) 涉及T细胞失调和转向T助手2反应,支持白血病B细胞存活.
干白素-25 (IL-25) 参与启动T助手2细胞反应,其受体IL-17RB对IL-25信号转导至关重要.
瘤微环境中IL-25的存在可能会影响周围瘤细胞的T细胞介导支持.

研究的目的:

为了研究介质素-25 (IL-25) 在慢性淋巴细胞白血病 (CLL) 的生物机制中的作用.
评估来自CLL患者和健康个体的T细胞和B细胞中IL-17RB的表达.
为了评估IL-25对T细胞激活和B细胞活力和CLL细胞亡的影响.

主要方法:

实时聚合酶连锁反应和流细胞测量被用来测量IL-17RB表达在外周血液单核细胞 (PBMCs) 从九个CLL患者和九个健康对照.
使用磁激活细胞分类 (MACS) 净化白血病B细胞.
用复合人体IL-25培养了PBMC和纯化的白血病B细胞,随后进行了MTT测定和AnnexinV/7AAD染色,以评估细胞活力和亡. 还测量了T淋巴细胞上的CD69表达和T和B细胞上的IL-17RB.

主要成果:

与健康个体相比,CLL患者的基础IL-17RB表达显著更高.
用IL-25培养增加了IL-17RB+ T细胞 (CD3+),IL-17RB+ B细胞 (CD19+) 和激活的T细胞 (CD69+) 的百分比,在CLL患者中比健康受试者更多.
IL-25治疗降低了CLL细胞的亡率,并刺激了T细胞,表明瘤细胞的存活率提高.

结论:

IL-25可能通过上调IL-17RB等与炎症相关的受体来促进CLL瘤细胞的存活.
在CLL患者中,IL-25似乎刺激T细胞并抑制B细胞死亡.
这些发现表明IL-25在慢性淋巴细胞白血病的发病和发展中可能发挥作用.

关键词:

: 慢性淋巴细胞白血病慢性淋巴细胞白血病介质素-25 介质素-25 介质素-25 帮助T细胞2细胞瘤微环境是一个微环境.

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