细胞死亡预测 (CellDeathPred):基于细胞绘画的铁和亡预测的深度学习框架
Kenji Schorpp1, Alaa Bessadok2, Aidin Biibosunov2
1Research Unit Signaling and Translation, Molecular Targets and Therapeutics Center, Helmholtz Zentrum München, Neuherberg, Germany.
Cell death discovery
|July 31, 2023
概括
细胞死亡的识别是具有挑战性的. 使用细胞绘画的深度学习框架CellDeathPred准确地区分铁和细胞死与健康细胞,准确度为95%.
科学领域:
- 生物医学成像学 生物医学成像学
- 计算生物学是一种计算生物学.
- 细胞生物学 细胞生物学
背景情况:
- 细胞死亡,包括亡和铁亡,在发育,恒常性和疾病发病过程中至关重要.
- 鉴定特定的细胞死亡类型是复杂的,需要计算密集的方法和昂贵的实验.
- 准细胞死亡途径为癌症和神经退行等疾病提供了治疗潜力.
研究的目的:
- 开发一种新的深度学习框架,CellDeathPred,用于区分铁亡,细胞亡和健康细胞.
- 为了利用高含量成像和细胞绘画技术进行自动细胞死亡分类.
- 为细胞死亡发现提供计算效率高,准确的方法.
主要方法:
- 利用深度神经网络将微观细胞绘画图像嵌入到歧视性潜伏空间中.
- 实施了一个监督的对比损失函数来优化深度学习模型.
- 训练并验证了CellDeathPred框架对人类HT-1080细胞的显微镜数据集进行培养和验证,诱导铁和亡.
主要成果:
- CellDeathPred成功地将ferroptotic和apoptotic细胞与健康对照细胞区分开来.
- 在非聚焦细胞绘画数据集上实现了95%的平均准确性.
- 证明了该框架在分类不同细胞死亡模式方面的能力.
结论:
- CellDeathPred提供了一种强大而准确的深度学习方法,用于使用细胞绘画进行细胞死亡分类.
- 该框架解决了识别细胞死亡类型的挑战,促进疾病研究和治疗开发.
- 支持人工智能驱动的图像分析在推动细胞死亡发现和了解疾病机制方面的潜力.
相关概念视频
01:30Overview of Cell Death
7.4K
Cell death is an essential process where the body gets rid of old or damaged cells. Cell proliferation and death need to be balanced, as an imbalance between the two may lead to cancer or autoimmune diseases.
Cell death was observed in the early 19th century, but there was no experimental evidence to prove it. In 1842, Carl Vogt first discovered cell death in a metamorphic toad; however, it was not termed ‘cell death.’ Scientists discovered different cell death pathways only in the...
Cell death was observed in the early 19th century, but there was no experimental evidence to prove it. In 1842, Carl Vogt first discovered cell death in a metamorphic toad; however, it was not termed ‘cell death.’ Scientists discovered different cell death pathways only in the...
7.4K
01:30Apoptosis
11.6K
Apoptosis is a combination of two Greek words, 'apo' and 'ptosis,' meaning separation and falling off, respectively. Hippocrates used this word to describe gangrene, which was caused due to bandaging of fractured bones. Apoptosis was distinguished from necrosis in 1970 when John Kerr reported observations of morphological changes occurring during apoptosis. During one experiment, he observed that the disruption of blood supply to the liver tissue resulted in a size...
11.6K
01:18Autophagic Cell Death
3.5K
Christian de Duve discovered “autophagy,” a process in which cellular components are engulfed by membrane-bound organelles called autophagosomes. The autophagosomes then fuse with lysosomes to digest the enclosed contents. Autophagy is generally activated in cells to prevent cell death. However, cell death is triggered when the damage is beyond repair.
Autophagy and Apoptosis
Autophagy can activate apoptosis. In normal conditions, the autophagy activating protein Beclin-1 and...
Autophagy and Apoptosis
Autophagy can activate apoptosis. In normal conditions, the autophagy activating protein Beclin-1 and...
3.5K
01:16Necrosis
4.6K
Necrosis is considered as an “accidental” or unexpected form of cell death that ends in cell lysis. The first noticeable mention of “necrosis” was in 1859 when Rudolf Virchow used this term to describe advanced tissue breakdown in his compilation titled “Cell Pathology”.
Morphological Manifestations of Necrosis
Necrotic cells show different types of morphological appearance depending on the type of tissue and infection. In coagulative necrosis, cells become...
Morphological Manifestations of Necrosis
Necrotic cells show different types of morphological appearance depending on the type of tissue and infection. In coagulative necrosis, cells become...
4.6K
01:17Phagocytosis of Apoptotic Cells
3.9K
Cells undergoing apoptosis form apoptotic bodies that must be removed immediately to prevent inflammation, autoimmune diseases, and necrosis. Phagocytosis is carried out by professional phagocytes such as macrophages or immature dendritic cells. Non-professional phagocytes such as epithelial cells and fibroblasts also take part in this process; however, they are not as effective as professional phagocytes.
Normal cells contain receptors that prevent them from being recognized...
Normal cells contain receptors that prevent them from being recognized...
3.9K
01:22iPS Cell Differentiation
2.7K
The ability of induced pluripotent stem cells or iPSCs to differentiate into most body cell types has stimulated repair and regenerative medicine research over the past few decades. iPSC-derived blood cells, hepatocytes, beta islet cells, cardiomyocytes, neurons, and other cell types can repair injuries or regenerate damaged tissue in diseases such as diabetes and neurodegenerative disorders.
2.7K