DkDTX5/MATE5的优先传输活动影响了鱼中不同的形成
Ying Liu1, Xin Wu1, Chenfeng Sun1
1National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, Huazhong Agricultural University, Wuhan, 430070, China.
Journal of integrative plant biology
|August 1, 2023
概括
在DkDTX5/MATE5转运器中的等位基变异解释了和非的红之间氨素 (PA) 积累的差异. 这个传送器.
科学领域:
- 植物生物化学 植物生物化学
- 果实质量遗传学 果实质量遗传学
- 代谢物运输机制的代谢物运输机制
背景情况:
- 益氨酸 (PAs) 是影响桃水果质量的关键代谢物.
- 在收性 (A) 类型和非收性 (NA) 类型之间存在PA积累的显著差异.
- 这些PA变异背后的分子机制在很大程度上是未知的.
研究的目的:
- 研究DTX/MATE蛋白在调解PA积累中的作用.
- 阐明PA在A型和NA型红之间变化的机制.
- 确定影响PA含量和水果质量的特定遗传因素.
主要方法:
- 对六种参与PA积累的DTX/MATE蛋白进行比较分析.
- 与PA水平相关的DkDTX5/MATE5表达模式分析.
- 在Xenopus laevis的卵细胞输送试验中,测定了基质特异性.
- 位点定向突变发生,以确定DkDTX5/MATE5.5中的关键功能残留物.
主要成果:
- 代基变异和DkDTX5/MATE5的优先传输显著影响PA积累.
- DkDTX5/MATE5表达水平与NA型水果中PA积累相关.
- DkDTX5/MATE5优先运输PA前体 (甲基素,基素,基素酸盐).
- 一个缺乏Ser-84的非功能DkDTX5/MATE5等位基因在A型鱼中占主导地位.
- -84对于DkDTX5/MATE5与PA前体结合至关重要.
结论:
- DkDTX5/MATE5载体功能和基变异是鱼类型之间的PA差异的主要驱动因素.
- 了解DkDTX5/MATE5的作用为培育改进的红品种提供了洞察力.
- 针对DkDTX5/MATE5功能可以增强水果的质量特征.
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