儿童维生素D:伪高维生素症
Lily Olayinka1,2, Izmarie Poventud-Fuentes1,2, Emily Garnett1,2
1Department of Pathology and Immunology, Baylor College of Medicine, Houston, Texas, USA.
Journal of clinical laboratory analysis
|August 1, 2023
概括
儿童的维生素D水平错误升高可能是由于免疫测试的变化造成的. 在测试之前,一个简单的离心步骤可以解决这些测量差异,确保准确的维生素D评估.
科学领域:
- 儿科内分泌学 儿科内分泌学
- 临床化学 临床化学
- 实验室医学 实验室医学
背景情况:
- 维生素D的毒性在儿童中不常见.
- 免疫检测方法可能会产生错误的25-基维生素D水平升高,这会给诊断带来挑战.
- 准确量化维生素D代谢物对儿科健康至关重要.
研究的目的:
- 为了呈现儿童病例的错误升高的25-氧维生素D.
- 用一种特定的免疫试验来调查25-基维生素D在同一天测量的显著变化.
- 在儿科样本中确定一种可靠的方法来准确量化维生素D代谢物.
主要方法:
- 分析了两个患有错误增加25-氧维生素D的儿科病例.
- 使用Abbott i2000SR免疫检测法测量了25-氧维生素D水平.
- 样品在离心后再次测试,并使用液体染色学-并联质谱法 (LC-MS/MS) 和不同供应商的仪器进行确认.
主要成果:
- 错误地提高25-基维生素D水平导致儿科患者不必要的进一步测试.
- 在Abbott i2000SR免疫试验中观察到25-基维生素D测量的同日显著变化.
- 用LC-MS/MS和不同供应商的测定验证了这些发现.
结论:
- 在样本测试之前实施离心步骤解决了25-基维生素D的测量变化.
- 样品的再离心证明在标准化维生素D水平测量方面是有效的.
- 这一简单的预分析步骤提高了儿童群体基于免疫测试的维生素D测试的可靠性.
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