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相关概念视频

Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

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Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
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Protein Dynamics in Living Cells01:19

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Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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Computed Tomography-guided Time-domain Diffuse Fluorescence Tomography in Small Animals for Localization of Cancer Biomarkers
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乳腺组织分析使用临床兼容的时间解析光和扩散反射 (TRF-DR) 系统.

Erica Dao1, Gabriela Gohla2,3, Phillip Williams2,3

  • 1Department of Physics & Astronomy, McMaster University, Hamilton, Canada.

Lasers in surgery and medicine
|August 1, 2023
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概括

这项研究开发了一种使用光学特性的快速乳腺组织分析系统. 该系统有效地将瘤与正常组织区分开来,显示了乳腺癌手术中手术内边缘评估的前景.

关键词:
乳腺癌 乳腺癌 乳腺癌分散的反射率是分散的反射率.光是一种光.时间解决的光光.组织分析 组织分析

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科学领域:

  • 生物医学光学 生物医学光学
  • 医学物理 医学物理
  • 癌症的诊断 癌症的诊断

背景情况:

  • 传统的乳腺组织组织病理学评估是耗时的.
  • 需要更快,临床上兼容的乳腺组织分析方法.
  • 术内评估可以改善乳腺癌手术中的患者结果.

研究的目的:

  • 开发一个时间效率高,临床兼容的乳腺组织分析系统.
  • 调查体内系统的潜力,以改善患者的治疗结果.
  • 使用光学特性区分瘤,纤维腺体和脂肪乳腺组织.

主要方法:

  • 使用了时间分辨率光和分散反射 (DA) 系统的组合.
  • 测量了包括光强度和寿命,DA,吸收和散射系数在内的光学参数.
  • 组织样本经过组织学处理并染色以进行成分分类.

主要成果:

  • 在瘤,纤维腺和脂肪组织之间观察到光学参数的统计学上显著差异.
  • 原和FAD光强度和寿命,DA和散射系数在不同组织类型之间存在显著差异.
  • NADH光寿命和吸收系数也显示了瘤和正常组织群体之间的显著差异.

结论:

  • 可观察到的光学参数差异支持该系统在临床乳腺分析方面的潜力.
  • 该系统显示了在手术期间快速边缘评估的前景.
  • 需要进一步研究混合组织成分和纤维脂肪分类.