面向时间分辨率的动态光散射显微镜:在高时间分辨率下检索粒子大小分布
Oscar Urquidi1, Natercia Barbosa1, Johanna Brazard1
1Department of Physical Chemistry, Sciences II, University of Geneva, 30, Quai Ernest Ansermet, 1211 Geneva, Switzerland.
The Review of scientific instruments
|August 1, 2023
概括
这项研究引入了一种新的动态光散射显微镜 (micro-DLS) 设置,用于快速的粒子大小表征. 该系统实现了小于100毫秒的时间窗口,大大提高了动态系统的时间分辨率.
科学领域:
- 材料科学 材料科学 材料科学
- 纳米技术纳米技术
- 物理化学 物理化学
背景情况:
- 动态光散射 (DLS) 对于溶液中纳米级粒子大小分析至关重要.
- 传统的DLS仅限于静态系统,阻碍了对时间演变现象的研究.
- 越来越需要使用具有更好的时间分辨率的DLS技术.
研究的目的:
- 开发和展示一个具有显著增强时间分辨率的DLS显微镜设置 (micro-DLS).
- 为了在小于100毫秒的时间窗口中实现精确的粒子大小表征.
- 为了将DLS应用扩展到暂时演变的系统.
主要方法:
- 实现一个DLS显微镜设置.
- 使用与时间相关的单光子计数来准确地记录光子到达时间.
- 从用户定义的时间窗口 (sub-100 ms) 构建自相关函数.
主要成果:
- 微DLS设置准确地描述了单模 (60-220 nm PS) 和多模 (20 nm LUDOX + 80 nm PS) 粒子大小分布.
- 在小于100毫秒的时间窗口内实现了高精度.
- 较短的时间窗口导致更窄的尺寸分布,表明子组件的分辨率.
结论:
- 开发的微型DLS系统为粒子大小测定提供了前所未有的时间分辨率.
- 这一进步使得DLS能够研究以前无法访问的系统中的动态过程.
- 增强的时间分辨率将扩大DLS应用范围,超越静态系统.
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