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Updated: Jul 20, 2025

Single-cell Analysis of Bacillus subtilis Biofilms Using Fluorescence Microscopy and Flow Cytometry

Single-cell Analysis of Bacillus subtilis Biofilms Using Fluorescence Microscopy and Flow Cytometry

Published on: February 15, 2012

在Bacillus subtilis生物膜中进行广泛的细胞多任务处理.

Sarah M Yannarell^1,2, Eric S Beaudoin³, Hunter S Talley²

¹Department of Microbiology and Immunology, University of North Carolina , Chapel Hill, North Carolina, USA.

|August 1, 2023

概括

细菌细菌生物膜显示复杂的细胞分化,许多细胞执行多种功能. 这挑战了以前的模型,并强调了对微生物群落进行更广泛研究的需要.

关键词:

生物膜是一种生物膜.细胞类型细胞类型同焦点显微镜的共焦显微镜.流动细胞计量是流动细胞计量的方法.光记者光记者

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相关实验视频

Last Updated: Jul 20, 2025

Single-cell Analysis of Bacillus subtilis Biofilms Using Fluorescence Microscopy and Flow Cytometry

Single-cell Analysis of Bacillus subtilis Biofilms Using Fluorescence Microscopy and Flow Cytometry

Published on: February 15, 2012

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Published on: October 29, 2016

Using Coculture to Detect Chemically Mediated Interspecies Interactions

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科学领域:

微生物学微生物学
系统生物学系统生物学
细菌遗传学细菌遗传学

背景情况:

细菌细菌形成生物膜,复杂的社区对于生存至关重要.
在生物膜中,细胞表现出多样化的表型,但这种异质性的程度尚不清楚.
现有的模型可能过于简化了B. subtilis.细胞分化和任务分配.

研究的目的:

研究B. subtilis生物膜中亚种群的空间组织和基因表达关系.
为了确定表型异质性的程度,并识别不同的细胞角色.
挑战和完善当前细菌生物膜中细胞分化的模型.

主要方法:

为14个关键基因创建了182个B. subtilis菌株,配有双向光转录记者.
聚焦显微镜可视化生物膜内的基因表达的空间分布.
流细胞计量量化记者共表达和识别多任务细胞.

主要成果:

空间映射揭示了生物膜内的不同基因的独特,有时是同定位的表达模式.
流细胞计表明了显著的共同表达,表明许多细胞同时执行多个功能.
只有少数子群体,如表面素/平酸生产者和分泌/有能力细胞,表现出不同的作用.

结论:

B. subtilis生物膜表现出比以前建模的更复杂的细胞分化.
专门从事单一任务的细胞的概念是一种过度简单化;许多细胞是多功能性的.
这些发现为了解微生物社区动态提供了一个框架,并对抗细菌感染的治疗策略产生影响.