脂质转移蛋白4调节棉花胺含量,并激活纤维细胞延长
Yujia Duan1,2, Xiaoguang Shang1,2,3, Qingfei He1,2
1State Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization, Nanjing Agricultural University, Nanjing 210095, China.
Plant physiology
|August 1, 2023
概括
棉花 棉花 棉花 棉花 棉花
科学领域:
- 植物生物学 植物生物学
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 细胞延长对于植物生长至关重要.
- 脂质代谢在细胞延长中的作用尚未完全理解.
- 了解这些机制是农业进步的关键.
研究的目的:
- 研究棉花脂质转移蛋白4 (GhLTP4) 在纤维细胞延长中的功能.
- 阐明GHLTP4影响细胞生长的分子机制.
- 确定涉及GHLTP4表达的调控因素.
主要方法:
- 在棉纤维中对 GhLTP4 的基因表达分析.
- 基因操纵 (过度表达和下调) 的 GhLTP4.
- 生物化学试验测量胺和auxin水平.
- 对辅酶响应基因表达的分析.
- 识别与GHLTP4促进体结合的转录因子.
主要成果:
- GhLTP4的表达在延长的棉纤维中最高.
- 过度表达GHLTP4通过增加胺含量和激活auxin通路来增加纤维长度.
- 低调GHLTP4减少了纤维的长度.
- 陶化物促进延长,其中印醇-3-酸 (IAA) 作用于下游.
- GhbHLH105转录因子积极调节GHLTP4的表达,影响球体发育和纤维长度.
结论:
- GhLTP4是棉纤维细胞延长的关键调节者.
- GhLTP4通过调节胺水平和激活辅酶信号来起作用.
- GhbHLH105-GhLTP4调节途径对于棉花芽的发育和纤维产量至关重要.
相关概念视频
Role of Microtubules in Cell Wall Deposition
2.4K
Microtubules are small hollow tubes in eukaryotic cells. The cell wall microtubules are polymerized dimers of two globular proteins, α-tubulin and β-tubulin, two globular proteins. With a diameter of about 25 nm, microtubules are the widest components of the cytoskeleton. They help the cell resist compression and provide a track along which vesicles move through the cell or pull replicated chromosomes to opposite ends of a dividing cell. Microtubules go through quick cycles of...
2.4K
Cellulose and Pectic Polysaccharides
3.7K
Every plant cell has a cell wall that protects the cell, provides structural support, and gives the cell shape. Cellulose, the main structural component of the plant cell wall, makes up over 30% of plant matter. It is the most abundant organic compound on earth. Cellulose is an unbranched polysaccharide composed of linear chains of glucose molecules linked by β (1→4) glycosidic bonds.
As a cell matures, its cell wall specializes according to its type. For example, the...
As a cell matures, its cell wall specializes according to its type. For example, the...
3.7K
C4 Pathway and CAM
45.7K
Most plants use the C3 pathway for carbon fixation. However, some plants, such as sugar cane, corn, and cacti that grow in hot conditions, use alternative pathways to fix carbon and conserve energy loss due to photorespiration. Photorespiration is the process that occurs when the oxygen concentration is high. Under such conditions, the rubisco enzyme in the Calvin cycle binds O2 instead of CO2, which halts photosynthesis and consumes energy.
C4 Pathway
The C4 pathway is used by plants such as...
C4 Pathway
The C4 pathway is used by plants such as...
45.7K
Cell Signaling in Plants
5.7K
Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
5.7K
Regulation of Nuclear Protein Sorting
2.4K
Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
2.4K
Mechanism of Lamellipodia Formation
2.6K
Cells migrating in response to external stimuli form lamellipodia, which are thin membrane protrusions supported by a mesh of linked, branched, or unbranched actin filaments. These actin filaments interact with myosin motor proteins, creating the dynamic actomyosin complex within the cytoskeleton. Contractility, or the ability to generate contractile stress, is inherent to the actomyosin complex. It helps cells detect the stiffness of the surrounding ECM and exert contractile force for...
2.6K


