密集结合的转录因子之间的结合合作的架构
Offir Lupo1, Divya Krishna Kumar1, Rotem Livne1
1Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel.
Cell systems
|August 1, 2023
概括
转录因子 (TF) 的结合特异性并不总是合作的. Msn2作为TF约束力的核心因素,但相互的TF合作很少见,挑战现有的模式.
科学领域:
- 分子生物学分子生物学
- 遗传学 是一个遗传学.
- 系统生物学 系统生物学
背景情况:
- 转录因子 (TF) 与基因组DNA结合是特定的,通常发生在首选的动机部位.
- 经常假设TF结合特异性涉及相互作用的TF的合作结合,但这在很大程度上仍未经测试.
- 了解TF依赖性对于破译基因调节至关重要.
研究的目的:
- 为了调查在芽酵母TFs与重叠的促进体结合的依赖关系.
- 测试合作TF相互作用在指导TF约束特异性的模型.
- 描述多个TFs共同消耗的全基因组后果.
主要方法:
- 在发芽酵母中多个TF的共同耗尽后,TF结合后果的全基因组概况.
- 分析TF依赖性和交互模式在重叠的促进者.
- 评估 Msn2 在其他 TFs 耗尽后的结合稳定性.
主要成果:
- 确定了单向的TF相互作用,Msn2成为使TF在其目标促进体结合成为可能的中心因素.
- 没有观察到相互TF合作的情况;即使在14个具有相似约束力的TF被共同填写时,Msn2的约束力仍然很强.
- TF的共同消耗的整体影响是适度和促进器特异的,不能完全解释非DNA结合区域在TF特异性中的作用.
结论:
- 该研究挑战了合作互动的普遍模式作为TF-binding特异性的主要驱动因素.
- Msn2在促进特定主办方的TF绑定方面发挥着关键的,非合作性的作用.
- 重新评估合作互动在指导TF约束偏好的作用是合理的.
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