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SNHG15有助于SARS-CoV-2通过RABL2A进入

Samuel Pushparaj^1,2, Chaitanya Gandikota^1,2, Kishore Vaddadi^1,2

¹Oklahoma Center for Respiratory and Infectious Diseases, Oklahoma State University, Stillwater, OK USA.

|August 2, 2023

概括

小核核RNA宿主基因15 (SNHG15) 促进严重急性呼吸系统综合征冠状病毒2 (SARS-CoV-2) 进入. 这种长长的非编码RNA通过与Rab-like蛋白2A (RABL2A) 相互作用来帮助病毒进入.

科学领域:

病毒学病毒学
分子生物学分子生物学
遗传学遗传学是一个

背景情况:

严重急性呼吸道综合征冠状病毒2 (SARS-CoV-2) 利用特定的宿主因子进入细胞.
虽然已知蛋白质因子,但长非编码RNAs (lncRNAs) 在SARS-CoV-2入口中的作用在很大程度上尚未被探索.

研究的目的:

调查小核核RNA宿主基因15 (SNHG15) 在SARS-CoV-2的进入机制中的参与.
阐明SNHG15在病毒进入过程中介导的分子相互作用和途径.

主要方法:

使用了一种SARS-CoV-2尖端伪型的lentivirus系统与光酶记者试验.
通过过度表达和淘汰技术操纵了SNHG15和Rab-like蛋白2A (RABL2A) 的表达水平.
评估了这些操纵对病毒进入效率的影响.

主要成果:

过度表达SNHG15显著增强了SARS-CoV-2的进入,而SNHG15的淘汰以剂量和时间依赖的方式抑制了它.
发现SNHG15与RABL2A相互作用,RABL2A是参与调节病毒进入的另一种蛋白质.
调节RABL2A的表达模仿了SNHG15对病毒输入的影响,而RABL2A倒置取消了SNHG15诱导的病毒输入增加.

结论:

SNHG15作为一个关键的调节因素,促进SARS-CoV-2进入宿主细胞.
SNHG15和RABL2A之间的相互作用对于SNHG15在促进病毒进入中的作用至关重要.

关键词:

在 RABL2A 中,RABL2A 是一个非常复杂的类型.这就是SARS-CoV-2病毒.在SNHG15中,SNHG15是第15个.宿主因素是宿主因素的组成部分.长非编码RNAs的编码.病毒进入病毒的入口

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