基于CRISPR的不同生物分子的诊断,从核酸,蛋白质和小分子到外体
Yuanshou Zhu1,2, Meng Zhang3, Shujuan Guo1
1Shanghai Center for Systems Biomedicine, Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Jiao Tong University, Shanghai 200240, China.
Acta biochimica et biophysica Sinica
|August 2, 2023
概括
通过将各种生物分子信号转化为核酸检测,CRISPR技术提供了多功能分子诊断. 这篇评论探讨了CRISPR.
科学领域:
- 分子生物学分子生物学
- 生物技术是生物技术.
- 诊断技术 诊断技术 诊断技术
背景情况:
- 基于CRISPR的检测是分子诊断的一个快速发展的领域.
- 一个关键的挑战是将来自各种生物分子 (核酸,蛋白质,小分子,外体,离子) 的信号转化为可检测的基于CRISPR的核酸信号.
- 现有的审查往往缺乏从不同目标生物分子的角度全面的概述.
研究的目的:
- 介绍用于分子诊断的CRISPR核酶的原理和特征.
- 总结和评估基于CRISPR检测各种生物分子的最新进展.
- 讨论一般检测方法与CRISPR系统的整合.
主要方法:
- 对基于CRISPR的分子诊断现有文献的审查和综合.
- 分析不同CRISPR核酶及其应用.
- 用于CRISPR检测的各种放大和信号读取策略的比较.
- 评估将不同生物分子信号转换为可检测CRISPR格式的方法.
主要成果:
- 对适用于分子诊断的CRISPR核酶的详细概述.
- 基于CRISPR的核酸,蛋白质,小分子,外体和离子检测方法的综合总结.
- 对放大和信号读取技术的比较分析,突出了与CRISPR集成的战略.
- 确定CRISPR诊断技术的当前局限性和未来方向.
结论:
- 克里斯普尔技术对广泛的目标进行分子诊断具有重大前景.
- 将各种检测原理与CRISPR系统集成,对于广泛应用至关重要.
- 需要在未来取得进展,以加强对定量,无放大,多重,全合一和临床检测 (POCT) 应用的CRISPR诊断.
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