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Updated: Aug 13, 2026

Efficient Production and Purification of Recombinant Murine Kindlin-3 from Insect Cells for Biophysical Studies
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[在Sf9昆虫细胞中包装的复合腺相关病毒6的随机集成分析]

M H Zhang1,2, X M Liu1,2,3, C Zhang1,2,4

  • 1School of Biomedical Engineering (Suzhou), Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, 230026 China.

Molekuliarnaia biologiia
|August 2, 2023
PubMed
概括

再组合腺相关病毒 (rAAV) 基因治疗可以随机集成到基因组中. 这项研究发现,rAAV6的整合是剂量依赖的,建议使用较低的剂量以获得更安全的基因治疗.

关键词:
在Baculo-Sf9制造平台上.反向嵌套的PCR是一种反向嵌套的PCR.随机整合 随机整合 随机整合再组合腺相关病毒.

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相关概念视频

Viral Recombination00:57

Viral Recombination

Cells are sometimes infected by more than one virus at once. When two viruses disassemble to expose their genomes for replication in the same cell, similar regions of their genomes can pair together and exchange sequences in a process called recombination. Alternatively, viruses with segmented genomes can swap segments in a process called reassortment.