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短时间的热冲击对介质干细胞的转录组有长期影响

Ivana Ribarski-Chorev¹, Gisele Schudy¹, Carmit Strauss¹

¹The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Rehovot 7610001, Israel.

|August 2, 2023

概括

热应激 (HS) 通过基因表达的持续变化改变介质干细胞 (MSC) 身份. 基因组基因修饰剂可能解释了短期的HS如何对细胞身份产生长期影响.

科学领域:

细胞生物学细胞生物学
分子生物学分子生物学
基因组学就是基因组学.

背景情况:

热应激 (HS) 对生理系统构成重大风险.
导致HS影响的分子机制,特别是对干细胞的影响,尚未完全理解.

研究的目的:

研究HS对介质干细胞 (MSC) 的影响.
阐明驱动MSC身份的HS诱导的改变的分子机制.

主要方法:

对MSC暴露于短期HS的转录组分析.
在HS恢复之前,期间和之后的基因表达特征的比较.
对双价基因激活和基因组修饰剂表达的分析.

主要成果:

HS显著改变了MSC的转录格局.
即使在短时间的HS暴露后,也观察到细胞特性的持续变化.
暂时上调MLL和基因组修饰剂与稳定的双价基因激活相关.

结论:

短期的HS可以诱导MSC身份的稳定,长期变化.
基斯基因修饰剂可能会调解HS对细胞身份的持续影响.
这些发现提供了预测和修改HS诱导的细胞变化的潜在策略.

关键词:

生物科学生物科学细胞生物学细胞生物学干细胞研究的研究.

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