相关实验视频
Updated: Jul 20, 2025
11:42
Induction of Mesenchymal-Epithelial Transitions in Sarcoma Cells
Published on: April 7, 2017
9.5K
在胃癌中,LINC00460通过与miRNA-143-5p竞争性结合来调解HMGA2的表达
Xuqing Zhu1, Yanli Xiang1, Feifei Mo1
1Department of Gastroenterology, Taizhou Municipal Hospital, Zhejiang, China.
Turkish journal of biology = Turk biyoloji dergisi
|August 2, 2023
概括
长非编码RNA LINC00460通过通过海绵miRNA-143-5p调节HMGA2促进胃癌. 这项研究增强了对胃癌发育中的长非编码RNA的理解.
科学领域:
- 分子生物学分子生物学
- 在瘤学瘤学.
- 遗传学 遗传学 是一个
背景情况:
- 胃癌 (GC) 的发展与长非编码RNAs (lncRNAs) 的异常表达密切相关.
- 不同表达的lncRNAs (DElncRNAs) 对GC的特定生物学影响仍然在很大程度上未被研究.
研究的目的:
- 研究LINC00460在胃癌中的作用和机制.
- 阐明涉及LINC00460,miRNA-143-5p和HMGA2在GC进展中的调节途径.
主要方法:
- 生物信息学分析用于差异表达和目标预测.
- 在体外测试包括MTT,殖民地形成和细胞增殖,迁移和入侵的Transwell测试.
- 双露西法酶记者测定和RNA免疫沉 (RIP) 来确认分子相互作用.
主要成果:
- 在GC组织和细胞中,LINC00460被显著上调,促进了增殖,迁移和入侵.
- LINC00460直接与miRNA-143-5p结合,HMGA2被确定为miRNA-143-5p的下游目标.
- LINC00460通过海绵化miRNA-143-5p促进了GC进展,从而提高了HMGA2表达的调节.
结论:
- 在胃癌中,LINC00460起到关键的瘤性 lncRNA 的作用.
- 该LINC00460/miRNA-143-5p/HMGA2轴在促进GC的扩散,入侵和迁移方面发挥着重要作用.
- 这提供了对 lncRNA 介导的 GC 开发背后的分子机制的新见解.
相关概念视频
02:39lncRNA - Long Non-coding RNAs
8.6K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
8.6K
01:22MicroRNAs
3.0K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.0K
02:23Master Transcription Regulators
6.9K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
6.9K
02:23Abnormal Proliferation
4.6K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.6K
02:33Hedgehog Signaling Pathway
7.4K
The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
7.4K