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博基拉:NGS读取模拟器可以消除序列分析中的读取核酸偏差

Ümit Akköse¹, Ogün Adebali^1,2

¹Faculty of Engineering and Natural Sciences, Sabancı University, İstanbul, Turkey.

Turkish journal of biology = Turk biyoloji dergisi

|August 2, 2023

概括

下一代测序 (NGS) 读数可能会因基因组核酸含量而产生偏差. 博基拉生成模拟序列来纠正这种偏差,改善各种应用的全基因组数据准确性.

科学领域:

基因组学就是基因组学.
生物信息学是一种生物信息学.
分子生物学分子生物学

背景情况:

全基因组的核酸含量本质上是异质的.
下一代测序 (NGS) 读数表现出受这种核酸分布影响的偏差.
这种偏见可能会影响全基因组分析的准确性.

研究的目的:

介绍Boquila,一个用于纠正NGS数据中基于核酸的偏差的新工具.
为了提高全基因组测序的准确性,读取分布.
为各种基因组应用中偏差校正提供灵活的解决方案.

主要方法:

博基拉产生合成序列,复制真实NGS读数的核酸概况.
该工具可以配置为专注于特定的基因组区域.
它结合了输入的DNA测序数据,以纠正副本数量变异.

主要成果:

博基拉有效地模仿了实际测序读数的核酸概况.
该工具在全基因组NGS数据中成功纠正基于核酸的偏差.
它提供了针对特定地区的灵活性,并包含副本数量变化的数据.

结论:

博基拉提供了一种强大的方法来缓解NGS数据中的核酸偏差.
该工具提高了全基因组测序研究的可靠性.
它与标准格式的兼容性有助于将其整合到现有的生物信息工作流中.

关键词:

没有NGS,没有NGS.核酸含量核酸含量序列阅读模拟的模拟

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